International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3319-3335

Diversity Analysis and Hybridity Test of Drought Responsive Wheat Cultivars and their F1’s on the Basis of SDS-PAGE Profiles
Usha Pant* and V.K. Khanna
Department of Genetics and Plant Breeding, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar- 263 145, India
*Corresponding author

The genetic polymorphism revealed by biochemical markers (protein and isozymes) in plant population have been extensively used to, assess the genetic variability, confirm hybridity of F1s, assure purity of cultivar and establish relationship between species. Electrophoretic protein analysis holds potential utility in breeding programmes of wheat through helping in the unequivocal characterization of varieties used. Sixteen wheat genotypes along with their 32 F1’s were characterized by using protein profile polymorphism in seeds through SDS-PAGE. The total number of bands varied from 9-17 with arrange of 3000 kd to 20,100 kd. The similarity coefficient ranged from 0.985-0.632 among parental lines. The dendrogram constructed on the basis of similarity coefficient clearly divided the genotypes into two major clusters. In most of the crosses the protein profile was more likely to the maternal parent with few bands from the male parent. Presence and/or absence of unique bands were also recorded in crosses which might be because of cis/trans regulation for gene expression and protein synthesis.

Keywords: Electrophoresis, protein polymorphism, SDS PAGE, Wheat

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How to cite this article:

Usha Pant and V.K. Khanna. 2018. Diversity Analysis and Hybridity Test of Drought Responsive Wheat Cultivars and Their F1’s on the Basis of SDS-PAGE Profiles.Int.J.Curr.Microbiol.App.Sci. 7(6): 3319-3335. doi:
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