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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3113-3118
DOI: https://doi.org/10.20546/ijcmas.2018.706.365


Development of Blast Resistant Dubraj by Introgression of Resistant Genes through Marker Assisted Selection
Shinde Umesh Dnyaneshwar1*, Toshy Agrawal1, Ashish Pradhan2, Tanvi Kadu1
Anil S. Kotasthane2 and Satish B. Verulkar1
1Department of Plant Molecular Biology and Biotechnology, COA, IGKV, Raipur (C.G.), India
2Department of Plant Pathology, COA, IGKV, Raipur (C.G.), India
*Corresponding author
Abstract:

Despite the fact that rising global population requires increased crop production, the rate of increase in crop yields is currently declining. The traits related to yield, stability and sustainability should be a major focus of plant breeding efforts. These traits include durable disease resistance, abiotic stress tolerance along with nutrient and water use efficiency. The use of DNA markers in plant breeding is called marker assisted selection (MAS). So far, about 101 major blast genes have been identified, many of them have been mapped cloned on different rice chromosomes, and tightly linked DNA markers have been developed. So, blast resistance genes can be combined using MAS in a single genetic background to develop rice cultivars with broad-spectrum durable resistance to blast. In this research work, we have introgressed two genes Pi1 and Pi2 in local variety of Chhattisgarh dubraj. Dubraj is extremely popular aromatic short medium grain variety in Chhattisgarh and Madhya Pradesh region. PR-122 is used as donor for resistant gene to introgress blast genes Pi-1 & Pi-2. The cross was attempted and progenies were obtained in Kharif 2016 season. Out of 29 plants 9 plants were selected for further screening. Progenies were screened phenotypically as well as genotypically for blast resistance for three consecutive seasons (Kharif 2016, Summer 2017 & Kharif 2017). During kharif 2017, 9 plants showing blast resistance both phenotypically as well as genotypically were sown in summer 2018. Forty seven plants with positive correlation between phenotype and genotype for blast resistance as observed in Summer 2017, were taken up for further generation advancement and screening during Kharif- 2018. Combination of conventional and marker assisted selection approach will provide opportunities for breeders to develop high yielding, stress tolerant and better quality rice variety.


Keywords: Rice, Molecular markers, marker assisted selection, Blast, Magnaporthe grisea
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How to cite this article:

Shinde Umesh Dnyaneshwar, Toshy Agrawal, Ashish Pradhan, Tanvi Kadu, Anil S. Kotasthane and Satish B. Verulkar. 2018. Development of Blast Resistant Dubraj by Introgression of Resistant Genes through Marker Assisted Selection.Int.J.Curr.Microbiol.App.Sci. 7(6): 3113-3118. doi: https://doi.org/10.20546/ijcmas.2018.706.365