International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3086-3095

Identification of Putative Sources of Tolerance to Aphid in Mustard and Generation of Molecular Marker for Use in Crop Improvement
Subhash Chander1*, Vikas C. Tyagi2, Deepali Sharma3, K. V. Bhat3 and Shashi Bhalla3
1ICAR-Directorate of Weed Research, Jabalpur, Madhya Pradesh 482004, India
2ICAR-Indian Grassland and Fodder Research Institute, Jhansi, UP, India
3National Bureau of Plant Genetic Resources, New Delhi 110 012, India
*Corresponding author

RAPD (random amplified polymorphic DNA) analysis was used to identify molecular markers linked to putative gene conferring resistance to the mustard aphid (Lipaphis erysimi Kalt.). A set of 30 germplasm accessions of Brassica juncea were screened with 284 RAPD primers (87 primers were amplified) for analysis of putative source of aphid resistance. These amplified primers were screened with bulked DNA of tolerant and susceptible accessions and from that four polymorphic primers were obtained. These four primers were screened with individual tolerant and susceptible accessions and only one RAPD primer (oligos-193, Genemed 10-mer) with clear cut polymorphism between tolerant and susceptible accessions was obtained. This polymorphic band of 600 bp found in susceptible was eluted from two accessions i.e. IC-491558 and IC-491564. DNA polymorphisms were converted in to SCAR (Sequence Characterized Amplified Region) primers (BJ 01 and BJ 02). These SCAR primers however could not provide unequivocal result since the association between marker and resistance was not always unidirectional. This indicates the need to further refine the marker identified by primer walking procedure.

Keywords: Brassica juncea, Lipaphiserysimi, Polymorphism, RAPD, SCAR

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How to cite this article:

Subhash Chander, Vikas C. Tyagi, Deepali Sharma, K. V. Bhat and Shashi Bhalla. 2018. Identification of Putative Sources of Tolerance to Aphid in Mustard and Generation of Molecular Marker for Use in Crop Improvement.Int.J.Curr.Microbiol.App.Sci. 7(6): 3086-3095. doi:
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