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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3038-3045
DOI: https://doi.org/10.20546/ijcmas.2018.706.357


Antagonistic Effect of Pseudomonas fluorescens on the Mycelial Growth and the Viability of the Sclerotia of Rhizoctonia solani in vitro and in Soil
Y. Sandhya1*, C. P. D. Rajan2 and M. Reddikumar1
1Department of Plant Pathology, S.V. Agricultural College, Tirupati (517502), A.P., India
2Regional Agricultural Research Station, Nellore (524003), A.P., India
*Corresponding author
Abstract:

Pseudomonas fluorescens isolates NLR-B1, NLR-B2, NLR-B3 were tested in dual culture against R. solani among which NLR-B3 isolate was found to be more antagonistic in vitro against Rhizoctonia solani than other two isolates. Sclerotial germination was tested after incubating in NLR-B3 bacterial suspension in the concentrations from 10-4 to 10-8 CFU/ml, there was no inhibition in sclerotial germination at 10 min incubation at all concentrations of bacterial suspension, whereas at 20 min incubation, inhibition of sclerotial germination was 73.33 per cent at 10-4 concentration, 43.33 per cent at 10-5 concentration, 30 per cent at 10-6 concentration and there was no inhibition of sclerotial germination at 10-7 and 10-8 concentrations. At the incubation period of 30 min. inhibition in sclerotial germination was 53.33 per cent at 10-4 concentration, 36.67 percent at 10-5 concentration, 30 per cent at 10-6 concentration. Similar inhibition of 13.33 percent was observed both at 10-7 and at 10-8 concentration. Per cent inhibition increased with increase in concentration of bacterial suspension at 20 and 30 min incubation periods.The bacterial suspensions at the earlier concentrations were added to the soil and incubated for 10 days after which the sclerotia were retrieved and tested for their viability, the inhibition of sclerotial germination was 56.67 per cent at 10-4 concentration, 36.66 per cent at 10-5 concentration and 16.66 per cent at 10-6 concentration. However at lower concentration of 10-7 and 10-8 there was no inhibition of sclerotial germination. There was a significant increase in per cent inhibition of sclerotial germination with increase in concentration of the bacterial suspension.


Keywords: Pseudomonas fluorescens, Rhizoctonia solani, Sclerotia, dual culture
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How to cite this article:

Sandhya Y., C. P. D. Rajan and Reddikumar M. 2018. Antagonistic Effect of Pseudomonas fluorescens on the Mycelial Growth and the Viability of the Sclerotia of Rhizoctonia solani in vitro and in Soil.Int.J.Curr.Microbiol.App.Sci. 7(6): 3038-3045. doi: https://doi.org/10.20546/ijcmas.2018.706.357