Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3002-3008
DOI: https://doi.org/10.20546/ijcmas.2018.706.353


Dynamics of Infection in Selected Tissues of White Spot Syndrome Virus-Infected Litopenaeus vannamei
K.Jeena1*, Rahul Krishnan2, K. U. Shyam2, P. Gireesh Babu3, W. S. Lakra4, C. S. Purushothaman5 and K. Pani Prasad1
1Aquatic Environment and Health Management Division, ICAR-Central Institute of Fisheries Education, Mumbai-61, India
2Department of Aqualife Medicine, Chonnam National University, Republic of Korea
3Fish Genetic and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Mumbai-61, India
4ICAR-Central Institute of Fisheries Education, Mumbai-61, India
5ICAR-Central Marine Fisheries Research Institute, Kochi-18, India
*Corresponding author
Abstract:

White spot syndrome virus (WSSV) remains as the most dreaded pathogen of shrimp aquaculture since its first incidence in China in 1991. WSSV is a double stranded DNA virus belonging to the genus Whispovirus of family Nimaviridae. It has a wide host range and because of its high virulence it can cause 100% mortality in a period of 3-10 days. The target tissues of WSSV are of ectodermal and mesodermal origin, including gills, cuticular epithelium etc. In the present study, tissue level dynamics of white spot syndrome virus infection in P. vannamei was investigated. For this healthy shrimps were challenged with the virus and a time course quantification of virus load in subcuticular epithelium, gills and pleopods was carried out by Real Time PCR against the generated standard curve, which could detect as minimum as 10 copies of virus in tissue samples. In the examined tissues, the viral load increased as time progressed, however, at different degrees. Compared with gill, viral load was higher in the sub-cuticular epithelium followed by the pleopods. The study provides knowledge regarding the infectivity within the early time periods following infection. This baseline information could potentially contribute in the sensitivity determination during development of diagnostic techniques with enhanced sensitivity which can help to manage the disease to a greater extent.


Keywords: WSSV, virus load, standard curve, Real Time PCR, L. vannamei
Download this article as Download

How to cite this article:

Jeena K., Rahul Krishnan, K. U. Shyam, P. Gireesh Babu, W. S. Lakra, C. S. Purushothaman and Pani Prasad K. 2018. Dynamics of Infection in Selected Tissues of White Spot Syndrome Virus-Infected Litopenaeus vannamei.Int.J.Curr.Microbiol.App.Sci. 7(6): 3002-3008. doi: https://doi.org/10.20546/ijcmas.2018.706.353