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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 2480-2486
DOI: https://doi.org/10.20546/ijcmas.2018.706.294


Marker Assisted Selection for Mapping Population against Chickpea Wilt
Renuka Tatte1*, S. S. Mane1, Snehal Jukte1, Pravin Jadhav2,S. Nandanwar3 and A. N. Patil4
1Department of Plant Pathology, Post Graduate Institute, Akola, Maharashtra, India
2Biotechnology Center, Post Graduate Institute, Akola, Maharashtra, India
3Department of Agril. Botany, Post Graduate Institute, Akola, Maharashtra, India
4AICRP on Pulses, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar Po, Akola (Ms) 444104, India
*Corresponding author
Abstract:

Marker assisted characterization of The 30 chickpea genotypes of chickpea F4 generation were selected for screening from three mapping population of different crosses (JG 62 (Susceptible) X ICCV 08113, PG 04154 (resistant) X JG 62 (susceptible) and PG 07101 (resistant) X JG 62 (susceptible). 10 genotypes from each population were selected for Fusarium wilt reaction was carried out using two molecular markers reported by earlier workers linked to disease resistant/susceptibility. In the present study, Allele Specific Associated Primer (ASAP) marker (namely, CS-27F/R700) linked to susceptibility and three Sequence Tagged Microsatellite site (STMS) marker (TA-59258, TA-96275, TR-19227) linked to resistance allele were validated. The results indicates that ASAP (CS 27F/CS 27R) marker linked to susceptibility alleles and susceptibility is dominant over resistance. ASAP marker, (CS27F/R) screened on the 30 genotypes and gave no amplified product of 700 bp. in all 30 genotypes STMS marker, TA-59, TA-96 and TR-19 linked to resistance and produce amplification in resistant genotypes. The amplification sizes of TA-59, TA-96 and TR-19 as characterized in Cicer sp. were 258, 275 and 227 bp, respectively. Amplification was observed in all 30 genotype.


Keywords: Chickpea, Fusarium wilt, Molecular markers, Resistance, Susceptible
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How to cite this article:

Renuka Tatte, S. S. Mane, Snehal Jukte, Pravin Jadhav, R. S. Nandanwar and Patil A. N. 2018. Marker Assisted Selection for Mapping Population against Chickpea WiltInt.J.Curr.Microbiol.App.Sci. 7(6): 2480-2486. doi: https://doi.org/10.20546/ijcmas.2018.706.294