National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Marker assisted characterization of The 30 chickpea genotypes of chickpea F4 generation were selected for screening from three mapping population of different crosses (JG 62 (Susceptible) X ICCV 08113, PG 04154 (resistant) X JG 62 (susceptible) and PG 07101 (resistant) X JG 62 (susceptible). 10 genotypes from each population were selected for Fusarium wilt reaction was carried out using two molecular markers reported by earlier workers linked to disease resistant/susceptibility. In the present study, Allele Specific Associated Primer (ASAP) marker (namely, CS-27F/R700) linked to susceptibility and three Sequence Tagged Microsatellite site (STMS) marker (TA-59258, TA-96275, TR-19227) linked to resistance allele were validated. The results indicates that ASAP (CS 27F/CS 27R) marker linked to susceptibility alleles and susceptibility is dominant over resistance. ASAP marker, (CS27F/R) screened on the 30 genotypes and gave no amplified product of 700 bp. in all 30 genotypes STMS marker, TA-59, TA-96 and TR-19 linked to resistance and produce amplification in resistant genotypes. The amplification sizes of TA-59, TA-96 and TR-19 as characterized in Cicer sp. were 258, 275 and 227 bp, respectively. Amplification was observed in all 30 genotype.
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