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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 1-16
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.001


Screening of Natural Bacterial Flora of Pomegranate Roots (Punica granatum L.) and their Antibiotic Activity in Taif, Saudi Arabia
Ahmad F. Shahaby1,2*, Abdulla A. Alharthi1 and Adel E. El Tarras1,2,3
1Biotechnology and Genetic Engineering Unit, College of Medicine, Taif University, Taif, Saudi Arabia
2Cairo University, College of Agriculture, Department of Microbiology, Genetics, Cairo, Egypt
3Al-Saedan Research Chair for Genetic Behavioral Disorders, Egypt
*Corresponding author
Abstract:

Taif region is situated in the central foothills of the Sarawat mountains at an altitude of 2200 to 2500 m above the sea level. Special type of pomegranate is cultivated there and called  taify pomegranate. Therefore, exploring the types of microorganisms tolerant to root exudates is important.  The objective of this study was to identify bacteria, actinomycetes and potential bacterial pathogens of pomegranate roots and their antimicrobial susceptibility patterns as well as effect of fruit parts extract on isolated bacteria. A total of 102 isolate were isolated and identified by morphological, physiological, APi profiles and 16S-rRNA techniques.  Out of 102 isolated bacteria, from free soil 38 (37.3%), rhizosphere 55(53.9%) and inside roots 9(8.8%). Isolated Actinomycetes represent 23 (22.5%), Streptomyces21 (20.6%) and Sacchromyces cerevieace 5(4.9%) of all isolated microorganisms. Isolates were identified as B. subtilis, S. cerevieace, S. pneumoniae, S. pyogenes, K. pneumoniae, A. baumannii, P. aerogenosa, A. chroococum, H. seropedicae,  A. lipoferumand E.aerogenes. Susceptibility testing was done according to Clinical and Laboratory Standards Institute (CLSI) guideline. Tetracycline was effective against 75.5% of isolated pathogens. In general, Gram positive isolates were more susceptible to all antibiotic tested, whereas Gram negative isolates were less susceptible to all antibiotic tested. Antimicrobial activity of crude extracts of rind, fruit, and juice ofpomegranate fruitswere evaluated against 18Gram-positiveand 35 Gram-negative bacteria. Crude alcoholic extracts of rind, seeds extract (juice) and whole fruit were evaluated against isolated Gram-positive and Gram-negative by agar disc diffusion method. All fruit parts were active against all tested bacteria (B. subtilis, E. aerogenes, K. pneumonia and P. aerogenosa), and moreover, against N2-Fixing bacteria (A. chroococcum, A.lipoferum and H. seropedicae). Rind extract   was less active against P. aeruginosa, juice against E. aerogenes, whole fruit extract against B. subtilis. In general, juice (seed extract) was relatively more active than extracts of other parts of the fruit. The descending order of activity of the extracted parts of the plant fruit was juice, rind and whole fruit, respectively. On the other hand, Gram- positive bacteria, B. subtilis S. pneumoniae and  A. chroococcum were more sensitive than Gram-negative and their inhibition zones. Therefore, it may be concluded that fruits of pomegranate extracts have broad spectrum antimicrobial activity and their activity could be attributed, to a great extent, to their contents of alkaloids and flavonoids.


Keywords: Pomegranate, isolation, Identification of bacteria, 16S-rRNA, Antibiotics, susceptibility, Fruit extracts
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How to cite this article:

Ahmad F. Shahaby, Abdulla A. Alharthi and Adel E. El Tarras. 2016. Screening of Natural Bacterial Flora of Pomegranate Roots  (Punica granatum L.) and their Antibiotic Activity in Taif, Saudi Arabia.Int.J.Curr.Microbiol.App.Sci. 5(2): 1-16. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.001