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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1994-2007
DOI: https://doi.org/10.20546/ijcmas.2018.706.236


Malassezia Species Associated With Dermatitis in Dogs and Their Antifungal Susceptibility
Uday Seetha, Sumanth Kumar, Raghavan Madhusoodanan Pillai, Mouttou Vivek Srinivas*, Prabhakar Xavier Antony and Hirak Kumar Mukhopadhyay
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Pondicherry-605 009, India
*Corresponding author
Abstract:

The present study was taken with the objective of isolation, characterization, molecular detection and antifungal sensitivity of Malassezia species from dermatitis cases from dogs in and around Pondicherry state. A total of 100 skin swabs were collected from the dogs showing dermatological problems suggestive of Malassezia. Out of 100 swabs, 41 Malassezia isolates were successfully isolated and had good growth on Sabouraud’s Dextrose Agar (SDA) during the primary isolation from the skin swabs. Biochemical tests for catalase, β-glucosidase activities and the capability to grow with three water soluble lipid supplements, namely Tween 20, Tween 80 and Cremophor EL concluded that the M. pachydermatis was the sole species isolated from the cases of canine dermatitis in Pondicherry state. Cytological examination revealed that direct skin swab smear was more sensitive than adhesive tape technique and impression smear. The frequency of isolation of M. pachydermatis was higher in neck region (8) followed by other regions in canine. Out of 100 skin swabs screened using an M. pachydermatis species specific primers, 61 were identified positive. The study showed a higher sensitivity of PCR (61%) in detecting Malassezia dermatitis over culture (41%). Based on invitro antifungal susceptibility studies it can be concluded that Ketoconazole, Itraconazole, Fluconazole and Amphotericin B can be advocated as the drug of choice to treat Malassezia dermatitis in this geographical location.


Keywords: Malassezia pachydermatis, Sabouraud’s dextrose agar, Modified Dixon’s agar, Polymerase chain reaction, Antifungal Susceptibility testing
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How to cite this article:

Uday Seetha, Sumanth Kumar, Raghavan Madhusoodanan Pillai, Mouttou Vivek Srinivas, Prabhakar Xavier Antony and Hirak Kumar Mukhopadhyay. 2018. Malassezia Species Associated With Dermatitis in Dogs and Their Antifungal Susceptibility.Int.J.Curr.Microbiol.App.Sci. 7(6): 1994-2007. doi: https://doi.org/10.20546/ijcmas.2018.706.236