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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1786-1795
DOI: https://doi.org/10.20546/ijcmas.2018.706.212


Role of Extracellular Proteases in Biofilm Degradation Produced from Escherichia coli and Pseudomonas mendocina
Poonam Kumari1*, Savita Jandaik2 and Sudha Batta3
1Department of Microbiology Shoolini University, Solan (H.P)-173212, India
2Department of Plant Pathology, University of Horticulture and Forestry, Nauni Solan(H.P)-173212, India
3Department of Microbiology Shoolini Institute of Life Sciences and Business Management, Solan, India
*Corresponding author
Abstract:

One of the most important properties of the protease enzyme is to degrade the biofilm because removal of biofilms is very difficult. In industrial settings, both the inactivation and removal of biofilms are of huge concern. If only disinfection without the removal of attached biofilms occurs, the inactivated biofilm cells may provide an ideal environment for further adhesion and growth, resulting in a complex matrix. Microbial resistance to biocides and their negative environmental impact are the main reasons for finding alternative biofilm control strategies. Enzymes are highly selective and capable of distrupting the structural stability of the biofilm EPS (extracellular polymeric substance) matrix. The biofilms acts as a barrier for antibiotics to affect the pathogenic bacteria and this may account for some people developing chronic Borreliosis/ Lyme symptoms. However proteolytic enzymes are very effective at dissolving mucupolysaccharides in biofilm. The 0.2 ml of purified alkaline protease of molecular weight of 29 kDa has been applied for degrading the biofilm of Pseudomonas or E.coli in 10 minutes. When biofilm was treated with the thermophilic protease enzyme the growth of bacteria producing biofilm was minimum (E.coli 0.230 at A 660 nm and Pseudomonas mendocina 0.120 at A 660nm). The combination of protease and polysaccharides was successful in biofilm removal or detachment of the organisms.


Keywords: Alkaline protease, Bacillus subtilis, Biofilm, Polysaccharide
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How to cite this article:

How to cite this article: Poonam Kumari, Savita Jandaik and Sudha Batta. 2018. Role of Extracellular Proteases in Biofilm Degradation Produced from Escherichia coli and Pseudomonas mendocina. Int.J.Curr.Microbiol.App.Sci. 7(06): 1786-1795. doi: https://doi.org/10.20546/ijcmas.2018.706.212Int.J.Curr.Microbiol.App.Sci. 7(6): 1786-1795. doi: https://doi.org/10.20546/ijcmas.2018.706.212