International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1492-1503

Characterization of a Bacteriocin from Bacillus amyloliquefaciens
Susan Brock, Judith Knadler, Tabitha Ritter and Jason C. Baker*
Missouri Western State University, St. Joseph, Missouri, USA
*Corresponding author

A potentially novel bacteriocin, currently named Bacocin, was discovered from a strain of Bacillus amyloliquefaciens. Bacocinsupernatant had a broad antibacterial spectrum, inhibiting Gram-positive and Gram-negative bacteria such as Escherichia coli, Staphylococcus and Salmonella species, and Listeria monocytogenes, but not against Enterococcusor Bacillus species. It significantly inhibited fungal growth of species such as Aspergillus, Stachybotrys, and Botrytis but not Rhizopus or Fusarium. It was stable at 121oC for 30 min and at 100oC for more than 1 hour. It maintained inhibitory activity through freeze/thaw and extreme pH conditions. Bacocin’s activity was not significantly destroyed by Proteinase K or Trypsin but was by high levels of Pronase E.Bacocin was purified from cell-free supernatant by ammonium sulfate precipitation, hydrophobic chromatography, and membrane size filtration. The stability and inhibitory spectrum of Bacocin makes it a good candidate for biological control of potential human, animal, and plant pathogens.

Keywords: Bacteriocin, antimicrobial peptide, Bacillus amyloliquefaciens, antibacterial, antifungal

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How to cite this article:

Susan Brock, Judith Knadler, Tabitha Ritter and Jason C. Baker. 2018. Characterization of a Bacteriocin from Bacillus amyloliquefaciens.Int.J.Curr.Microbiol.App.Sci. 7(6): 1492-1503. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.