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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1460-1467
DOI: https://doi.org/10.20546/ijcmas.2018.706.173


Isolation and Characterization of Rhizobium meliloti Isolated from Rhizosphere Soil and Roots of Fenugreek from Different Locations
Satyadev Prajapati1*, M. S. Dadke2, S. Surekha2, S. Godika2 and V. Prasanna Krishna2
1Department of Plant Pathology, S.K.N. COA, SKNAU, Jobner, Jaipur (Rajasthan)-303329, India
2College of Agriculture, Latur, VNMKV, Parbhani, India
*Corresponding author
Abstract:

The nine isolates of Rhizobium meliloti were isolated on CR-YEMA medium from root nodules and rhizosphere soil of fenugreek. Four isolates showed absorption of Congo red and rest five isolates did not absorbed Congo red. All the isolates showed similar cultural characteristic for colony colour and shape except for colony size. Colonies of all isolates were whitish, translucent, sticky nature, rounded and had a diameter in the range of 3.2 to 5.2 mm. All the isolates showed positive reaction for biochemical test viz., catalase test, potassium hydroxide and starch hydrolysis test and showed negative reaction for Gram’s staining.


Keywords: Rhizobium meliloti, root nodules, rhizosphere soil, fenugreek, cultural characters, biochemical test
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How to cite this article:

Satyadev Prajapati, M. S. Dadke, S. Surekha, S. Godika and V. Prasanna Krishna. 2018. Isolation and Characterization of Rhizobium meliloti Isolated from Rhizosphere Soil and Roots of Fenugreek from Different Locations.Int.J.Curr.Microbiol.App.Sci. 7(6): 1460-1467. doi: https://doi.org/10.20546/ijcmas.2018.706.173