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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 862-872
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.097


Pathogenic Bacteria Prevalence in a Selected Environmental Sample and their Sensitivity to Routine Antibiotics
Ndirika D. Nnabue1, Michael U. Orji1, Amechi S. Nwankwegu1* and Chinwe J. Aneke2  
1Department of Applied Microbiology and Brewing, Faculty of biosciences, Nnamdi Azikiwe University PMB, 5025, Awka, Anambra State, Nigeria
2Department of Biochemistry, Enugu State University of science and Technology, ESUT, Enugu, Nigeria
*Corresponding author
Abstract:

The prevalence of human pathogenic bacteria especially Salmonella species in cow dung as a selected environmental sample was investigated. Five different abattoirs in Anambra State, Nigeria were used. Potentially pathogenic organisms were isolated and identified and they include; Salmonella enteritidis, Shigella sp., Clostridium perfringens, Pseudomonas sp., E. coli, Staphylococcus aureus, Vibrio cholerae and Vibrio parahemolyticus. The susceptibility of the isolates to different antibiotics was tested and it was observed that Pseudomonas sp. was very sensitive to the antibiotics, Ceporex (10µg) and Tarivd (10µg) with 16mm zone of inhibition. Clostridium sp. was more susceptible to Levofloxacin (20µg) with  20mm zone of inhibition, Staph. aureus was more susceptible to Streptomycin (30µg) and Levoflaxacin (20µg) with  18mm zone of inhibition, E. coli was more susceptible to Gentamycin (10µg) with 20mm zone of inhibition, Salmonella enteritidis was more sensitive to Augmentin (30µg) with 20mm zone of inhibition and Shigella sp. was more sensitive to Ciproflox (10µg) with 20mm zone of inhibition. The pathogenicity of these isolates was studied by infecting each on mice. There was death of two mice infected with Clostridium perfringens .Mice infected with Shigella sp., suffered swollen of the scrotum and scrotal sac which was observed after dissection. Mice infected with Pseudomonas sp., Staphylococcus aureus, and Salmonella enteritidis and E. coli, 25x108, 8x108, 20x108, 10x108 cfu/ml of the infected organisms were recovered from their intestine respectively. This shows that the organisms colonized their intestine at high level and they shed them in their faeces, though the infections were asymptomatic at the stage.


Keywords: Prevalence,cow dung, pathogenic bacteria, Antibiotics, sensitivity.
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How to cite this article:

Ndirika D. Nnabue, Michael U. Orji, Amechi S. Nwankwegu and Chinwe J. Aneke. 2016.  Pathogenic Bacteria Prevalence in a Selected Environmental Sample and their Sensitivity to Routine AntibioticsInt.J.Curr.Microbiol.App.Sci. 5(8): 862-872. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.097