International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1420-1432

Regeneration of Sugarcane (Saccharum officinarum L.) Clones from Mutagenic Treated Calli
S. J. Mallikarjuna*, M. Hemath Kumar, P. Sudhakar and D. Mohan Reddy
Department of Genetics and Plant Breeding, S. V. Agricultural College, Tirupati, India
*Corresponding author

An experiment was carried out at the Agricultural Research Station, Perumallapalle for the regeneration of somaclones. Two sugarcane clones, 2008T42 (susceptible to red rot) and 2009T5 (susceptible to smut) were used as plant material. Calli from the controls and different concentrations of mutagenic chemicals (sodium nitrite and EMS) were transferred on to MS media with 3 mg l-1 BAP, 2 mg l-1 IAA and 2 mg l-1 kinetin for evaluating the effect on shooting. In various treatments, o.6 µM EMS in 2009T5 (T10) exhibited good response for shoot induction as evident by minimum time taken for shoot initiation, more shoot number, frequency and shoot length. Full grown shoots were transferred on to rooting medium with 3 mg l-1 NAA for initiation and elongation of roots to study the effect of mutagenic chemicals on root induction. 0.6 µM EMS in 2009T5 (T10) was found to be better for root induction among all the treatments. It has produced more number of roots with higher length of root in less time.

Keywords: Sodium nitrite, EMS, Leaf rolls, Regeneration, Somaclones

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How to cite this article:

Mallikarjuna S. J., M. Hemath Kumar, P. Sudhakar and Mohan Reddy D. 2018. Regeneration of Sugarcane (Saccharum officinarum L.) Clones from Mutagenic Treated Calli.Int.J.Curr.Microbiol.App.Sci. 7(6): 1420-1432. doi:
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