International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1404-1411

Effects of Different Mutagenic Chemicals on Callogensis in Sugarcane (Saccharum officinarum) Clones 2008T42 and 2009T5
S. J. Mallikarjuna*, M. Hemath Kumar, D. Mohan Reddy and P. Sudhakar
Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati-517502, India
*Corresponding author

Unexpanded leaf rolls were used as explants for callus induction with different concentrations of mutagenic chemicals (sodium nitrite and ethylmethane sulfonate). The effect of these mutagenic chemicals on callusing, shooting and rooting was studied. Callus induction was observed in different concentrations of sodium nitrite (3 mg l-1, 5 mg l-1 and 7 mg l-1) and EMS (0.6 µM, 0.8 µM, 1.0 µM) along with controls. Among all the treatments 3 mg l-1 (T4) SN and 0.6 µM EMS (T10) in 2009T5 were found to be the best for callus induction traits. The treatments responded with minimum number of days for callus initiation, maximum number of explants induced callus with high callus induction frequency and high callus size.

Keywords: Sodium nitrite, EMS, Leaf rolls, Calllus induction

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How to cite this article:

Mallikarjuna S. J., M. Hemath Kumar, D. Mohan Reddy and Sudhakar P. 2018. Effects of Different Mutagenic Chemicals on Callogensis in Sugarcane (Saccharum officinarum) Clones 2008T42 and 2009T5.Int.J.Curr.Microbiol.App.Sci. 7(6): 1404-1411. doi:
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