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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1312-1324
DOI: https://doi.org/10.20546/ijcmas.2018.706.154


Callus Mediated In vitro Regeneration of Naga Chilli (Capsicum chinense Jacq.): The Fiery Pepper from North East India
G. Bora1, 2*, H. K. Gogoi1 and P. J. Handique2
1Division of Biotechnology, Defence Research Laboratory, Tezpur-784001, Assam, India
2Department of Biotechnology, Gauhati University, Guwahati-781014, India
*Corresponding author
Abstract:

Naga chilli or Bhut jolokia is extensively cultivated all over northeast India including Assam. This chilli is of high commercially value due to its pungent trait called capsaicin. The chilli is well known to have extreme potential for numerous pharmaceutical applications apart from being used for culinary purposes. Despite being such an important spice crop, demand of Naga chilli is deteriorating owing to natural cross pollination and mutation which in turn reduce its capsaicin content. Therefore, an attempt was made to conserve the germplasm through standardization of an efficient micropropagation protocol using different plant hormones, silver nitrate (AgNO3) and tryptophan as the key components. In vitro propagation was carried out with leaf explants. MS medium was supplemented with different combinations of BAP, 2,4-D, GA3, NAA and IBA alongside AgNO3 and tryptophan. Maximum callus induction took place in the presence of 2,4-D (3.5mg/L), AgNO3 (3mg/L) and trptophan (3mg/L). Addition of AgNO3 to the culture medium greatly enhanced shoot proliferation in the selected in vitro plantlets. In vitro flower induction and fruit formation were successfully carried out in the presence of GA3 (3.5mg/L) and AgNO3 (3.5mg/L). Maximum root initiation was observed in the MS medium supplemented with IBA (3mg/L), GA3 (2.5mg/L) and AgNO3 (3mg/L). Hardening of in vitro raised plantlets was successful in sterile soil enriched with vermicompost and vermiculite. Percentage of plant survival was 84.75%. Addition of biofertilizers greatly improved the chances of acclimatization of in vitro raised healthy plantlets.


Keywords: Naga chilli, In vitro propagation, Silver nitrate, tryptophan
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How to cite this article:

Bora G., H. K. Gogoi and Handique P. J. 2018. Callus Mediated In vitro Regeneration of Naga Chilli (Capsicum chinense Jacq.): The Fiery Pepper from North East India.Int.J.Curr.Microbiol.App.Sci. 7(6): 1312-1324. doi: https://doi.org/10.20546/ijcmas.2018.706.154