International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1254-1268

Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model
A. Hanaa - Mansour1, Maha G. Soliman2, Wedad A. Hasan1, Rasha A. El - Sayed2 and Nahla A. Hassaan2 ⃰
1Department of Pharmacology, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
2Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt
*Corresponding author

Acute respiratory distress syndrome (ARDS) is among the most common causes of respiratory failure in critically ill patients. Despite extensive research, ARDS remains a major cause of morbidity and mortality, with no effective pharmacological treatment options. The present study was conducted to evaluate the efficacy of bone marrow-derived stem cells (BM-MSCs) compared with Dexamethasone (Dex) and Sodium bicarbonate (NaHCO3) against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Rats were injected with 20µl of LPS of Escherichia coli in each nostril for two consecutive days. Treatments were carried out by administration of BM-MSCs (1 × 106 cells/rat), Dexamethasone (2mg/kg/i.p.) once before induction with LPS and sodium bicarbonate (10-15 ml/day for two consecutive days, while last dose was given 30 minutes before LPS inhalation). Treatment with BM-MSCs significantly decreased inflammatory influx, total leukocyte count in peripheral blood, reduction in oxidative stress, reversed depleted GSH in lung tissue, reduced inflammatory responses in rat nasal airways as detected by mucin rMuc-5AC gene expression in lung tissue when compared with LPS group. Moreover, BM-MSCs displayed signs of lung recovery and the severity of lung injury was significantly reduced when compared to the LPS group and restoring the normal histological picture of lung as well as apoptosis.

Keywords: Acute lung injury, Mesenchymal stem cells, Dexamethasone, Lipopolysaccharide, rMuc-5AC mRNA gene

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How to cite this article:

Hanaa - Mansour, A., Maha G. Soliman, Wedad A. Hasan, Rasha A. El-Sayed and Nahla A. Hassaan. 2018. Impact of Mesenchymal Stem Cells on Lipopolysaccharide-Induced Apoptosis in Acute Lung Injury Rat Model.Int.J.Curr.Microbiol.App.Sci. 7(6): 1254-1268. doi:
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