International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 1190-1197

Determination of Genetic Diversity among Sclerotium rolfsii Isolates Causing Collar Rot of Chickpea Using Simple Sequence Repeat (SSR) Markers
Epsita Swain1*, Archana A. Gadekar1, S.S. Mane1 and Jhumishree Meher2
1Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India
2Department of Plant Pathology, Jawaharlal Nehru Krishi Viswa Vidyalaya, Jabalpur, Madhya Pradesh, India
*Corresponding author

Six isolates of chickpea collar rot fungus of Sclerotium rolfsii were collected from different locations of Maharashtra were investigated for genetic diversity under present experiment. We employed five SSR of MB- series to construct a genotype-specific DNA fragment profile of field isolates of this fungus. The PCR amplified product of each primer was resolved on 10 % Polyacrylamide gel electrophoresis. The 5 SSR primers screened produced a total of 60 reproducible and scorable amplicons. The size of amplicons produced ranged from 115bp to 800bp. The percentage polymorphism generated by the SSR markers was 77.6% among all the 6 isolates of Sclerotium rolfsii. The result from the UPGMA based dendrogram generated for Sclerotium rolfsii isolates revealed that they were divided into two main clusters, Cluster-A and B which were further subdivided into sub-clusters. The isolates in the two clusters have an overall Jaccard's similarity coefficient of 35%. The similarity coefficient range varied from 0.27 to 0.83. The SSR markers suggest that there is genetic differentiation among the population.

Keywords: Chickpea, Sclerotium rolfsii, PAGE, Dendrogram, PCR, SSR marker

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How to cite this article:

Epsita Swain, Archana A. Gadekar, S.S. Mane and Jhumishree Meher. 2018. Determination of Genetic Diversity among Sclerotium rolfsii Isolates Causing Collar Rot of Chickpea Using Simple Sequence Repeat (SSR) Markers.Int.J.Curr.Microbiol.App.Sci. 7(6): 1190-1197. doi:
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