International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 959-967

In Silico Analysis and Molecular Docking Studies of Cajanus cajan Lectin against Aminopeptidase-N Receptor from Acyrthosiphon pisum
Rakesh Kumar Prajapat1, Puja Singh1, Poonam Tiwari1, Pawan Mainkar1, Sarika Sahoo2, A.R. Rao2 and Rekha Kansal1*
1ICAR-National Research Center for Plant Biotechnology, Pusa, New Delhi-12, India
2ICAR- Indian Agricultural Statistics Research Institute, Pusa New Delhi-12, India
*Corresponding author

Cajanus cajan lectins (CCL) is a promising candidate molecules for the protection against sap sucking insect pests like Lipaphis erysimi (Kaltenbach). Legume lectin toxicity against hemipteran has been proven by various experiments. This study explains the insecticidal potential of CCL through molecular modeling and docking with receptor alanyl Aminopeptidase N from Acyrthosiphon pisum membrane.The functional domain analysis of CCL revealed metal binding and N-linked glycosylation site. The physico-chemical parameter like theoretical isoelectric point (pI) is 5.59, instability index is 24, aliphatic index is 80.80 and GRAVY value is -0.070. The secondary structure attributes of CCL protein explain its structural behavior and biological activity.The CELLO2GO tools revealed subcellular localization of CCL protein was plasma membrane with a reliability score of 2.289. The present study using various bio-computational tools could, therefore, help in our understanding of CCL protein structure and prove to potential candidate gene for generating transgenic for increased aphid resistance.

Keywords: Cajanus cajan, Lectins, Functional domain, Molecular docking, Molecular modelling

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How to cite this article:

Rakesh Kumar Prajapat, Puja Singh, Poonam Tiwari, Pawan Mainkar, Sarika Sahoo, A.R. Rao and Rekha Kansal. 2018. In Silico Analysis and Molecular Docking Studies of Cajanus cajan Lectin against Aminopeptidase-N Receptor from Acyrthosiphon pisum.Int.J.Curr.Microbiol.App.Sci. 7(6): 959-967. doi:
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