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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 819-823
DOI: https://doi.org/10.20546/ijcmas.2018.706.096


Comparison of Different Media for Germ Tube Production by Candida albicans: A Retrospective Study
Aditi Mehta*, Mritunjay Kumar, Upasana Bhumbla, Anamika Vyas and A.S. Dalal
Department of Microbiology, Geetanjali Medical College and Hospital, Udaipur, India
*Corresponding author
Abstract:

Systemic candidiasis is associated with a high crude mortality rate, even with first line antifungal therapy. C. albicans is the predominant cause of invasive fungal diseases which is a serious public health issue. The main objective is to assess the reliability of different media for germ tube production in Candida albicans isolated from various clinical samples. All Candida isolates were identified and speciated by conventional methods such as Gram’s staining, germ tube test, chlamydospore formation on corn meal agar, sugar fermentation test, sugar assimilation test, and growth on Hi-chrome candida agar. Out of 128 clinical isolates of Candida albicans, 5 different methods were used for germ tube production. Pooled human sera showed 121/128 (94.53%) was the most sensitive method wherein YEPD (Yeast Extract Peptone Dextrose) Broth 104/128 (81.25%) was the reliable and easy method for detection of germ tube, followed by Trypticase soy broth 93/128 (72.65%); Peptone water 79/128 (61.71%) and 2% Sucrose 76/128 (59.37%).


Keywords: Germ tube production, Candida albicans, Retrospective study
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How to cite this article:

Aditi Mehta, Mritunjay Kumar, Upasana Bhumbla, Anamika Vyas and A.S. Dalal. 2018. Comparison of Different Media for Germ Tube Production by Candida albicans: A Retrospective Study.Int.J.Curr.Microbiol.App.Sci. 7(6): 819-823. doi: https://doi.org/10.20546/ijcmas.2018.706.096