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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 747-755
DOI: https://doi.org/10.20546/ijcmas.2018.706.087


Expression of Xa21 Allele Resistant to Bacterial Blight under Artificial Epiphytic Condition in Indian Basmati rice (Oryza sativa L.)
Surya Kant Mishra1, Navneet Kumar1, Pooran Chand1, Mukesh Kumar2, Devi Singh1 and Rahul Kumar3
1Molecular Biology Laboratory, Department of Genetics and Plant Breeding, SVP University of Agriculture and Technology, Meerut (U.P.)-250110, India
2Department of Horticulture, SVP University of Agriculture and Technology, Meerut (U.P.)-250110, India
3Department of Genetics and Plant Breeding, CCS University, Meerut, UP-200005, India
*Corresponding author
Abstract:

A bacterial blight (BB) resistance gene Xa21 linked to pTA248 molecular marker was transferred into Pusa Basmati-1 (IPB-1) to develop improved Pusa Basmati-1 (IPB-1). The bacterial culture Xanthomonas oryzae pv. oryzae (Xoo) was used to create artificial epiphytotic condition. The leaf samples were taken from PB-1 and IPB-1 treated with bacterial blight culture at different time interval (0 hour, 6 hour, 12 hour, 24 hour, 36 hour, 48 hour, 60 hour and 72 hour) to observe level of expression. RNA isolation and purification was carried out by TRIzol method and analyzed quantity of the total RNA for cDNA preparation. The 2-ΔΔCT method was used to analyse the relative changes in gene expression from real-time quantitative PCR. The normalized expression indicated that the variety IPB-1 carrying gene Xa21 expressed higher fold changes than that of susceptible variety PB-1 and control (0 hour) at tillering as well as adult stage. The adult stage expressed higher fold change in 2-ΔΔCt values than that of the tillering stage. However, the 2-ΔΔCt value of 72 hour at adult stage indicated that the expression of Xa21 allele is down-regulated after 60 hours in artificial epiphytic condition.


Keywords: Xanthomonas oryzae pv. oryzae, BB resistance, Quantitative real time PCR, Oryza sativa var. indica
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How to cite this article:

Surya Kant Mishra, Navneet Kumar, Pooran Chand, Mukesh Kumar, Devi Singh and Rahul Kumar. 2018. Expression of Xa21 Allele Resistant to Bacterial Blight under Artificial Epiphytic Condition in Indian Basmati rice (Oryza sativa L.).Int.J.Curr.Microbiol.App.Sci. 7(6): 747-755. doi: https://doi.org/10.20546/ijcmas.2018.706.087