National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A bacterial blight (BB) resistance gene Xa21 linked to pTA248 molecular marker was transferred into Pusa Basmati-1 (IPB-1) to develop improved Pusa Basmati-1 (IPB-1). The bacterial culture Xanthomonas oryzae pv. oryzae (Xoo) was used to create artificial epiphytotic condition. The leaf samples were taken from PB-1 and IPB-1 treated with bacterial blight culture at different time interval (0 hour, 6 hour, 12 hour, 24 hour, 36 hour, 48 hour, 60 hour and 72 hour) to observe level of expression. RNA isolation and purification was carried out by TRIzol method and analyzed quantity of the total RNA for cDNA preparation. The 2-ΔΔCT method was used to analyse the relative changes in gene expression from real-time quantitative PCR. The normalized expression indicated that the variety IPB-1 carrying gene Xa21 expressed higher fold changes than that of susceptible variety PB-1 and control (0 hour) at tillering as well as adult stage. The adult stage expressed higher fold change in 2-ΔΔCt values than that of the tillering stage. However, the 2-ΔΔCt value of 72 hour at adult stage indicated that the expression of Xa21 allele is down-regulated after 60 hours in artificial epiphytic condition.
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