International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 769-84

Screening of Antifungal Potential of Rhizospheric Isolates against Alternaria Leaf Blight Disease of Bt-cotton in vitro
Laxman S. Raut1* and Venkat S. Hamde2
1Department of Microbiology, Sant Tukaram Arts and Science College,Parbhani-431401, Maharashtra, India
2Department of Microbiology, Yogeshwari Mahavidyalaya, Ambajogai -431517,Maharashtra, India
*Corresponding author

Alternaria leaf blight is one of the most important yield reducing diseases of Bt-cotton caused by Alternaria macrospora. In order to find bio-based ecofriendly effective control measure to this phytopathogen, 114 rhizospheric bacterial isolates were screened for in vitro antifungal potential against Alternaria leaf blight phytopathogen using modified dual culture method. Out of 114 isolates tested, 18 rhizospheric bacterial isolates inhibited growth of Alternaria macrospora in vitro. Highest antifungal activity was exhibited by isolate RLS19 (91.43 %) and followed by RLS52 (88.57 %), RLS18 (85.71 %), RLS102 (85.71 %), RLS76 (82.86 %), RLS96 (82.86 %) in dual culture technique against the phytopathogen. Based on inhibition activities in dual culture assay, 8 efficient rhizospheric bacterial isolates were selected for further investigation. For characterizations of microbial control mechanism, rhizospheric bacterial isolates were tested for production of volatile metabolites, diffusible metabolites and siderophore. Volatile metabolite production was detected by double plate assay. Rhizospheric bacterial isolate RLS76 hamper 17.77 % radial growth by producing volatile metabolites against phytopathogen while other isolates were unable to produce volatile metabolites. Diffusible metabolite production was detected by agar well diffusion method. Rhizospheric bacterial isolates RLS19 and RLS76 showed highest (71.43 %) inhibition of phytopathogen by producing diffusible metabolites followed by RLS18 (68.57.00 %), RLS52, RLS56, RLS96, RLS102 (65.71 %) and RLS53 (57.14 %). Siderophore production ability was detected qualitatively by modified CAS agar plate method. All the tested, 8 rhizospheric bacterial isolates were able to produce siderophore. The highest siderophore was recorded by RLS18, RLS53 and RLS58.

Keywords: Bt-cotton, Alternaria leaf blight,Alternaria macrospora,volatile metabolites, diffusible metabolites and siderophore.

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How to cite this article:

Laxman S. Raut and Venkat S. Hamde. 2016. Screening of Antifungal Potential of Rhizospheric Isolates against Alternaria Leaf Blight Disease of Bt-cotton in vitroInt.J.Curr.Microbiol.App.Sci. 5(8): 769-84. doi:
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