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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 490-497
DOI: https://doi.org/10.20546/ijcmas.2018.706.054


Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers
S.E. Navyashree1, Sumangala Koulagi1, Sandhyarani Nishani2,V. Kantharaju3 and Chaya P. Patil4
1Department of Plant Pathology
2Department of Biotechnology and Crop Improvement, K. R. C. College of Horticulture, Arabhavi, Karnataka, India
3Department of Plant Pathology and Head AICRP on Fruits, KRCCH, Arabhavi, Karnataka, India
4KRCCH, Arabhavi, University of Horticultural Sciences, Bagalkot-587104, India
*Corresponding author
Abstract:

Twenty five isolates of Trichoderma harzianum and Trichoderma viride were obtained from rhizosphere soil of turmeric and ginger collected from Belagavi and Bagalkot districts of Karnataka and eight potential isolates based on antagonistic activity were studied using RAPD PCR. The genetic relatedness among two isolates of Trichoderma harzianum and six isolates of Trichoderma viride were analysed with five random primers. RAPD profiles showed genetic diversity among the isolates with the formation of two major clusters. Analysis of dendrogram revealed that similarity coefficient ranged from 0.15 to 0.95. Molecular variability among the potential isolates showed 81 amplified bands out of which 77 were polymorphic and 4 were monomorphic.


Keywords: Trichoderma harzianum, Trichoderma viride, RAPD, Genetic diversity, PCR, Dendrogram
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How to cite this article:

Navyashree, S.E., Sumangala Koulagi, Sandhyarani Nishani, V. Kantharaju and Chaya P. Patil. 2018. Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 7(6): 490-497. doi: https://doi.org/10.20546/ijcmas.2018.706.054