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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 368-375
DOI: https://doi.org/10.20546/ijcmas.2018.706.041


Determination of EC50 of Aqueous Extract of Dolichos biflorus Seeds against Ethylene Glycol Induced Renal Stone in Wistar Rats
Azhar Shuaib Batoo1*, Sahadeb Dey1, Mir Mudasir2, Zamir Ali Ganaie3, Afaq Amin Najar4 and Burhan Nabi4
1Division of Veterinary Medicine, Indian Veterinary Research Institute, Izatnagar-243122, India
2Division of Veterinary Pathology, G.B.P.U.A.T, Pantnagar, Uttrakhand-263145, India
3Division of Veterinary Parasitology, SKUAST - Kashmir-180004, India
4Division of Veterinary Medicine, SKUAST - Jammu-181102, India
*Corresponding author
Abstract:

The present study was undertaken to study the EC50 of aqueous extract of Dolichos biflorus seeds against ethylene glycol induced renal stone in rats. Hyperoxaluria and Calcium oxalate deposition in the kidney was induced by oral administration of ethylene glycol (EG) and ammonium chloride in the drinking water to a final concentration of 0.75% and 2%, respectively for 28 days. The animals were divided into 5 groups, viz. healthy control, untreated control, 150, 250 and 300 mg test extract groups. Efficacy of treatment was assessed by estimating serum concentrations of urea nitrogen, Creatinine and histopathology. The serum urea nitrogen and serum creatinine was lower in extract treated rats than the untreated rat. Histopathology of kidney revealed lesions in the renal parenchyma of untreated rats and kidney lesions were almost absent in 300 mg/kg body weight dose. The aqueous extract of Dolichos biflorus seeds was found to be effective against renal stone in wistar rats. The EC50 of the extract was found to be 300mg/kg body weight.


Keywords: Dolichos biflorus, Ethylene glycol, Histopathology, Renal parenchyma
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How to cite this article:

Azhar Shuaib Batoo, Sahadeb Dey, Mir Mudasir, Zamir Ali Ganaie, Afaq Amin Najar and Burhan Nabi. 2018. Determination of EC50 of Aqueous Extract of Dolichos biflorus Seeds against Ethylene Glycol Induced Renal Stone in Wistar Rats.Int.J.Curr.Microbiol.App.Sci. 7(6): 368-375. doi: https://doi.org/10.20546/ijcmas.2018.706.041