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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 182-192
DOI: https://doi.org/10.20546/ijcmas.2018.706.023


Evaluation of the Role of Culture, PCR, and Sequencing in the Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Ascites
Hazim Abdel-Wahab, Mahamoud Shokry, Sahar Abo-Eleyoun, Mohammed Sayed* and Shaimaa Hassouna
Department of Microbiology and Immunology, Faculty of Medicine, Minia University, Egypt
*Corresponding author
Abstract:

Spontaneous bacterial peritonitis (SBP) means the development of peritonitis (infection in the abdominal cavity) despite the absence of an obvious source of infection. It occurs almost exclusively in people with portal hypertension, usually as a result of liver cirrhosis, our aim of this study was detection of bacterial infections in SBP by conventional method and molecular biology. (19.5%) ascitic fluid (AF) samples were culture positive. PCR results show that bactDNA was detected in 56 samples (28%), 18 samples were sequenced for molecular identification of the amplified bactDNA fragments. 10 out of 18 were undetermined, while 6 were E. coli and 2 were Streptococci. Our conclusion is that the detection of bacterial DNA in the ascitic fluid provides an important role in diagnosis of SBP.


Keywords: SBP, Conventional method and molecular biology
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How to cite this article:

Hazim Abdel-Wahab, Mahamoud Shokry, Sahar Abo-Eleyoun, Mohammed Sayed and Shaimaa Hassouna. 2018. Evaluation of the Role of Culture, PCR, and Sequencing in the Diagnosis of Spontaneous Bacterial Peritonitis in Patients with Ascites.Int.J.Curr.Microbiol.App.Sci. 7(6): 182-192. doi: https://doi.org/10.20546/ijcmas.2018.706.023