National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Diagnosis of Female Genital tuberculosis (FGTB) is difficult using conventional diagnostic methods. This study was conducted to evaluate the use of polymerase chain reaction (PCR) in diagnosis of definitive and probable Female Genital tuberculosis and to assess the performance of insertion sequence (IS) 6110 based PCR assay as compared to conventional culture by Lowenstein-Jensen (L.J), Liquid Culture (MGI-960) methods for the diagnosis of FGTB. This study was conducted with one hundred and twenty six endometrial samples with the definite PCR and microbiological diagnosis of genital tract tuberculosis. Diagnosis of extra pulmonary tuberculosis is often difficult, because of the lower sensitivity and specificity of the methods as well as the invasive procedure of acquiring biopsy specimens. Of the 126 endometrial biopsy tissues specimens from highly-probable FGTB patients (based on clinical features), 27, 32 and 41 were positive by L.J (21.43%), MGIT (25.40%) and PCR (32.54%) respectively, whereas microscopy results were negative. PCR offered more sensitive in determining tuberculosis etiology in female infertility than any other conventional method in the diagnosis of clinically suspected Female Genital Tuberculosis.
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