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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 731-740
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.082


Molecular Epidemiology of Female Genital Tuberculosis leading to infertility
B. Usharani1, M. Muthuraj2*, B. Radhakrishnan3, Jothi Bobly James3,S. Govindarajan4 and K.V. Raman5
1Department of Genetics, Dr.A.L.M Post Graduate Institute for Medical Sciences,University of Madras, Chennai, Tamil Nadu, India
2Department of Microbiology, Intermediate Reference laboratory, State TB Training and Demonstration Centre, Govt. Hospital for Chest Diseases, Puducherry, India
3Rajiv Gandhi Government Women and Child Hospital, Puducherry, India
4State TB Cell, Government Chest Clinic, Puducherry, India
5Department of Health and Family Welfare Services, Government of Puducherry,
Puducherry, India
*Corresponding author
Abstract:

Diagnosis of Female Genital tuberculosis (FGTB) is difficult using conventional diagnostic methods. This study was conducted to evaluate the use of polymerase chain reaction (PCR) in diagnosis of definitive and probable Female Genital tuberculosis and to assess the performance of insertion sequence (IS) 6110 based PCR assay as compared to conventional culture by Lowenstein-Jensen (L.J), Liquid Culture (MGI-960) methods for the diagnosis of FGTB. This study was conducted with one hundred and twenty six endometrial samples with the definite PCR and microbiological diagnosis of genital tract tuberculosis. Diagnosis of extra pulmonary tuberculosis is often difficult, because of the lower sensitivity and specificity of the methods as well as the invasive procedure of acquiring biopsy specimens. Of the 126 endometrial biopsy tissues specimens from highly-probable FGTB patients (based on clinical features), 27, 32 and 41 were positive by L.J (21.43%), MGIT (25.40%) and PCR (32.54%) respectively, whereas microscopy results were negative. PCR offered more sensitive in determining tuberculosis etiology in female infertility than any other conventional method in the diagnosis of clinically suspected Female Genital Tuberculosis.


Keywords: Female Genital Tuberculosis, Infertility, Polymerase Chain Reaction, Endometrial tissues.
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How to cite this article:

Usharani, B., M. Muthuraj, B. Radhakrishnan, Jothi Bobly James, S. Govindarajan and Raman, K.V. 2016. Molecular Epidemiology of Female Genital Tuberculosis leading to infertility.Int.J.Curr.Microbiol.App.Sci. 5(8): 731-740. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.082