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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 15-21
DOI: https://doi.org/10.20546/ijcmas.2018.706.003


Tilling and Eco-Tilling - A Reverse Genetic Approach for Crop Improvement
Azra Khan1*, Ishfaq Abidi3*, M.A. Bhat1, Z.A. Dar2, Gowhar Ali3, Asif B. Shikari4 and M.A. Khan1
1Division of Genetics and Plant Breeding, Faculty of Agriculture Wadura, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
2Division of Genetics and Plant Breeding, Dryland Agriculture Research Station, Budgam, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
3Division of Genetics and Plant Breeding, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, India
4MCRS Sagam, Sher-i-Kashmir University of Agricultural Sciences and Technology of Kashmir, India
*Corresponding author
Abstract:

Targeting Induced Local Lesions in Genome (TILLING) is a general reverse technique that combines chemical mutagenesis with PCR based screening to identity point mutations in regions of interest. The strategy works with a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms in gene of interest. The main principles of TILLING include making EMS-mutagenized population, targeting gene of interest with PCR primers and searching rare mutants among many individuals. A study based on applying TILLING to elucidate gene function in a chemically induced sorghum mutant population showed that total of five mutants were detected for four gene targets. High throughput TILLING was demonstrated applicable to maize, with a large genome but with limited genetic resources currently available. This study also concluded public TILLING service for maize, modelled on the existing Arabidopsis TILLING Project. The basic applications of ECO-TILLING include mapping, association analysis, mutation profiling and biodiversity. The main advantage of TILLING is its ability in determining the spectrum of variation and for genetic mapping based on linkage association analysis. The technique is a however cost effective too. One of the basic disadvantages of TILLING is the low rate of induction of mutation and requirement of skilful labours.


Keywords: TILLING, ECO-TILLING, Mutagenesis, DNA polymorphism, Induced
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How to cite this article:

Azra Khan, Ishfaq Abidi, M.A. Bhat, Z.A. Dar, Gowhar Ali, Asif B. Shikari and Khan, M.A. 2018. Tilling and Eco-Tilling - A Reverse Genetic Approach for Crop Improvement.Int.J.Curr.Microbiol.App.Sci. 7(6): 15-21. doi: https://doi.org/10.20546/ijcmas.2018.706.003