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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 708-720
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.080


Molecular Identification and Characterization of the Biosurfactant Produced by Pseudomonas aeruginosa-PSPA15 from the Oil Contaminated Soil
P. Saminathan1* and P. Rajendran2
 1Research Scholar, Department of Microbiology, Karpagam University,Coimbatore- 641021, Tamil Nadu, India
2Professor, Department of Microbiology, Sri Ramachandra Medical College,Porur, Chennai, Tamil Nadu, India
*Corresponding author
Abstract:

Oil contaminating in the soil will lead to serious pollution problems. Bioremediation involves the use of organisms to remove pollutants from a contaminated site. Biosurfactant are structurally diverse compounds mainly produced by hydrocarbon utilizing microorganisms which makes them potential candidates for enhancing oil recovery. Pseudomonas aeruginosa PSPA15 strain is isolated from oil contaminated soil. It is screened to confirm the ability in the biosurfactant production. The surface tension value is 26mNm-1 and the emulsification indices are 83%, 79%, and 77% with petrol, diesel and kerosene respectively. The sequence of the 16srRNA and PSPA15 are compared and it shows 100% similarity to Pseudomonas aeruginosa gene bank. Biocompatibility assay exhibited no cytotoxicity against VERO cells. HPLC analysis shows retention time of 4.37 representing the rhamnolipids type of biosurfactant.


Keywords: Oil pollution, Bioremediation, Biosurfactant, Biocompatibility, High performance Liquid chromatography
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How to cite this article:

Saminathan, P., and Rajendran, P. 2016. Molecular Identification and Characterization of the Biosurfactant Produced by Pseudomonas aeruginosa-PSPA15 from the Oil Contaminated Soil.Int.J.Curr.Microbiol.App.Sci. 5(8): 708-720. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.080