National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Blackgram (Vigna mungo (L.) Hepper) (2n=22) is one of the most highly valuable pulse crop, cultivated in almost all parts of India. It is a good source of easily digestible proteins, carbohydrates and other nutritional factors. Besidesvarious biotic and abiotic constraints, viral diseases mostly yellow mosaic disease is the prime threat for extensive economic losses in areas of production. The Yellow Mosaic disease (YMD) caused by Mungbean Yellow Mosaic Virus (MYMV), a Gemini virus transmitted by whitefly (Bemesia tabaci Genn) is one of the most downfall disease that has the ability to cause yield loss upto 85%. The advancements in the field of biotechnology and molecular biology such as marker assisted selection and genetic transformation can be utilized in developing MYMV resistance uradbeans. The investigation was carried out to find out the markers linked to yellow mosaic virus resistance gene, MYMV resistant parent T9 and MYMV susceptible parent LBG 759 were crossed to produce mapping population. A total of 50 SSR primers were used to study parental polymorphism. Of these 14 SSR markers were found polymorphic showing 28% of polymorphism between the parents. These fourteen markers were used to screen the F2 populations to find the markers linked to the resistance gene by bulk segregant analysis. The marker CEDG185 present on linkage group 8 clearly distinguished resistant and susceptible parents, bulks and ten F2 resistant and susceptible plants indicating that this marker is tightly linked to yellow mosaic virus resistance gene.
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