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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 3810-3817
DOI: https://doi.org/10.20546/ijcmas.2018.702.451


Identification of Molecular Markers Linked to Yellow Mosaic Virus Resistance in Blackgram (Vigna mungo (L.) Hepper)
E. Rambabu*, Ch. Anuradha, V. Sridhar and S. Sokka Reddy
Institute of Biotechnology,Department of Molecular Biology and Biotechnology, College of Agriculture, Professor Jayashankar Telangana State Agriculture University, Rajendranagar, Hyderabad-500030, India
*Corresponding author
Abstract:

Blackgram (Vigna mungo (L.) Hepper) (2n=22) is one of the most highly valuable pulse crop, cultivated in almost all parts of India. It is a good source of easily digestible proteins, carbohydrates and other nutritional factors. Besidesvarious biotic and abiotic constraints, viral diseases mostly yellow mosaic disease is the prime threat for extensive economic losses in areas of production. The Yellow Mosaic disease (YMD) caused by Mungbean Yellow Mosaic Virus (MYMV), a Gemini virus transmitted by whitefly (Bemesia tabaci Genn) is one of the most downfall disease that has the ability to cause yield loss upto 85%. The advancements in the field of biotechnology and molecular biology such as marker assisted selection and genetic transformation can be utilized in developing MYMV resistance uradbeans. The investigation was carried out to find out the markers linked to yellow mosaic virus resistance gene, MYMV resistant parent T9 and MYMV susceptible parent LBG 759 were crossed to produce mapping population. A total of 50 SSR primers were used to study parental polymorphism. Of these 14 SSR markers were found polymorphic showing 28% of polymorphism between the parents. These fourteen markers were used to screen the F2 populations to find the markers linked to the resistance gene by bulk segregant analysis. The marker CEDG185 present on linkage group 8 clearly distinguished resistant and susceptible parents, bulks and ten F2 resistant and susceptible plants indicating that this marker is tightly linked to yellow mosaic virus resistance gene.


Keywords: Yellow Mosaic Virus Resistance, Blackgram (Vigna mungo (L.) Hepper)
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How to cite this article:

Rambabu E., Ch. Anuradha, V. Sridhar and Sokka Reddy S. 2018. Identification of Molecular Markers Linked to Yellow Mosaic Virus Resistance in Blackgram (Vigna mungo (L.) Hepper).Int.J.Curr.Microbiol.App.Sci. 7(2): 3810-3817. doi: https://doi.org/10.20546/ijcmas.2018.702.451