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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3522-3532
DOI: https://doi.org/10.20546/ijcmas.2018.705.407


Enhanced Saccharification in Sugarcane Leaves by Combined Pretreatment of Ionic Liquid and Aqueous Ammonia
Archana Singh, Ashutosh Dubey, Vivek Chandra Verma, Brijesh Lekhak and A.K. Verma*
Department of Biochemistry, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantanagar, Uttarakhand, India
*Corresponding author
Abstract:

Total recovery of fermentable sugar from lignocellulosic biomass has foremost problem for liquid biofuel production. Several pretreatment methods have been tried, however use of ionic liquids is one of promising method then others, but it also has less efficiency. Hence a method was developed for pretreatment of sugarcane leaves using ionic liquid EmimAc (1-ethyl-3-methylimidazolium acetate), aqueous ammonia and a combination of both. The combined use of ionic liquid and aqueous ammonia in 1:1 for pretreatment at 6 hrs followed by cellulase treatment showed a synergistic effect on sugarcane leaves with release of 90% glucose. While pretreatment with ionic liquid alone and aqueous ammonia alone followed by cellulase treatment produced 70% and 45% of glucose respectively. The ionic liquid was successfully recycled to minimize the cost, up to 6 times with efficiency of 87% release of glucose. Compared with the conventional pretreatment and other methods, this combined method is more efficient and cost effective.


Keywords: Lignocellulose, Pretreatment, Saccharification, Ionic liquid, Biodigestibility, Biofuel
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How to cite this article:

Archana Singh, Ashutosh Dubey, Vivek Chandra Verma, Brijesh Lekhak and Verma, A.K. 2018. Enhanced Saccharification in Sugarcane Leaves by Combined Pretreatment of Ionic Liquid and Aqueous Ammonia.Int.J.Curr.Microbiol.App.Sci. 7(5): 3522-3532. doi: https://doi.org/10.20546/ijcmas.2018.705.407