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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 3388-3398
DOI: https://doi.org/10.20546/ijcmas.2018.705.396


Comparative Evaluation on Phenolic Content and Antioxidant Activity of Legume Sprouts as Affected by Various Solvents for Application in Livestock Products
Serlene Tomar1, Geeta Chauhan1*, Annada Das2 and Med Ram Verma3
1Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P.-243122, India
2Department of Livestock Products Technology, Faculty of Veterinary Science, WBUAFS, Kolkata-700037, India
3Division of Livestock Economics and Information Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P.-243122, India
*Corresponding author
Abstract:

Oxidation process is one of the chief methods for producing free radicals in food, drugs and even living systems. Legumes are a source of not only essential nutrients required for life but also has a number of bioactive compounds required for the sustenance of good health and prevention of diseases. Germination is well known to significantly improve the bioavailability of nutrients and increase the phytochemical content of legumes. In the present study four sprouted legumes (T1- mung gram, T2- black gram, T3-pigeon pea and T4- Cowpea) were extracted in three different solvent systems (HWE, 60% EHWE and 60%MHWE) and investigated for their total phenolic content (TPC) and in-vitro antioxidant activity using different 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging, ABTS assay and reducing power assay. The sprouted legumes were also investigated for their Vitamin C content and total carotenoid content. The results revealed that 60% EHWE was best suited for extraction of TPC for T3 and T4 while 60% MHWE was better suited for T1 and T2 while 60% EHWE exhibited highest DPPH radical scavenging activities and reducing power assay for T4, T3 and T2 while 60% MHWE proved to be a better solvent for T1 while 60% MHWE exhibited highest ABTS scavenging activities for T1, T3 and T4. HWE was the least potent amongst all the solvent systems tested. T4 showed the highest TPC and antioxidant activities although no correlation was obtained between the TPC and antioxidant activities. The results of the study reveal that solvents with different polarity had significant effects on TPC and in-vitro antioxidant activities. This information may be of interest to the food industry as legumes are cheap sources of nutrients, bioactive compounds and sprouting is an inexpensive technique to improve their functional value which can be exploited for prevention of lipid oxidation in food systems.


Keywords: Comparative evaluation, Phenolic content, Antioxidant activity, Legume sprouts
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How to cite this article:

Serlene Tomar, Geeta Chauhan, Annada Das and Med Ram Verma. 2018. Comparative Evaluation on Phenolic Content and Antioxidant Activity of Legume Sprouts as Affected by Various Solvents for Application in Livestock Products.Int.J.Curr.Microbiol.App.Sci. 7(5): 3388-3398. doi: https://doi.org/10.20546/ijcmas.2018.705.396