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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 635-643
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.071


Prevalence and Antibiotic Susceptibility Pattern of Multi-drug Resistant Klebsiella pneumoniae in Pus samples from Surgical Ward Patients in a Tertiary Care Hospital in South India
Md. Mudassar Iqbal Lodhi1* and Shobha D. Nadagir2
 1Department of Microbiology, ICARE Institute of Medical Sciences and Research and Dr. B. C. Roy Hospital, Haldia, India
2Department of Microbiology, Navodaya Medical College Hospital and Research Centre, Raichur, India
*Corresponding author
Abstract:

Klebsiella pneumoniae, being prevalent in the nosocomial environment, is capable of expressing a variety of resistance enzymes, thus justifying their continued monitoring. The present study was undertaken to determine the prevalence of multi-drug resistant Klebsiella pneumoniae isolated from post surgical wound infection and to know their resistance pattern. Consecutive, non-repeat Klebsiella pneumoniae isolates obtained from post surgical wound infection were processed by conventional culture method and antimicrobial susceptibility test. ESBL, AmpC, MBL and KPC production was detected by Phenotypic confirmatory disk diffusion test, AmpC Disk test, Imipenem+EDTA Combined disk test and Modified Hodge Test respectively. Out of 84 pus samples, 67 (79.7%) were ESBL producers, 36 (42.8%) were AmpC producers and 12 (14.3%) were MBL producers. Thirty one (36.9%) Klebsiella pneumoniae isolates were both ESBL and AmpC co-producers whereas ESBL, AmpC and MBL co-producing isolates were 12 (14.3%). However none of the isolates were positive for Klebsiella pneumoniae Carbapenemase (KPC) by Modified Hodge Test. ESBL, AmpC and MBL positive isolates were more drug resistant compared to non-producers for all the antibiotics used in the study. Enhanced infection control, coupled with antibiotic stewardship programs, therefore play an important role in limiting the spread of this terrifying antimicrobial resistance.


Keywords: ESBL,AmpC,MBL,KPC,Klebsiella pneumoniae.
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How to cite this article:

Md. Mudassar Iqbal Lodhi and Shobha D. Nadagir. 2016. Prevalence and Antibiotic Susceptibility Pattern of Multi-drug Resistant Klebsiella pneumoniae in Pus samples from Surgical Ward Patients in a Tertiary Care Hospital in South IndiaInt.J.Curr.Microbiol.App.Sci. 5(8): 635-643. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.071