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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2590-2598
DOI: https://doi.org/10.20546/ijcmas.2018.705.299


Attribution of Biochemical Characters in Downy Mildew Resistant and Susceptible Hybrids of Pearl Millet
Pooja Sangwan, Kushal Raj*, L.K. Chugh, Annu and Annie Khanna
Department of Plant Pathology, CCS Haryana Agricultural University, Hisar Pin 125 004 (Haryana), India
*Corresponding author
Abstract:

Downy mildew, caused by Sclerospora graminicola (Sacc.) Schroet, is a disease of great economic significance in the production of pearl millet (Pennisetum glaucum). Host physiology particularly host metabolites and oxidising enzymes get disturbed due to attack of Sclerospora graminicola. Altered state of oxidative enzyme plays an important role in plant metabolism during pathogenesis. In the present investigation biochemical characterization of pearl millet genotypes - HHB 223 and 7042 S was carried out at 30, 40, 50 and 70 DAS. Total phenols were higher in resistant genotype at 30 DAS in comparison to susceptible genotype but with the progression of disease total phenols decreased in resistant genotype at 40, 50 and 70 DAS but in susceptible genotype they increased. Orthodihydroxy phenol was higher in resistant genotype at all the stages in comparison to susceptible genotype. Flavanols were higher in resistant genotypes at 30 DAS in comparison to susceptible genotype. With the progression of the disease, flavanol content decreased in both the genotypes but more reduction was observed from 30 DAS to 70 DAS in susceptible genotype. Total chlorophyll and carotenoid content decreased with the progression of systemic infection in both resistant and susceptible genotypes. Total soluble sugars were higher in resistant genotype in comparison to susceptible genotype. Peroxidase and catalase activities were higher in susceptible genotype at early stages of growth but at later stages higher in resistant genotype. Polyphenol oxidase did not show any significant difference in resistant and susceptible genotype. Phenylalanine Ammonia Lyase activity was higher and continuously increased in susceptible genotype at all stages of growth whereas Tyrosine Ammonia Lyase activity was found in traces in both the genotypes. Protein content was initially higher in susceptible genotype at 30 DAS and 40 DAS but it decreased with disease progression. Change in the activities of antioxidant enzymes and in various metabolites can be used as a biochemical marker for the degree of resistance.


Keywords: Downy mildew, Pearl millet, Peroxidase, Catalase, Phenol, Flavanol, Total chlorophyll
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How to cite this article:

Pooja Sangwan, Kushal Raj, L.K. Chugh, Annu and Annie Khanna 2018. Attribution of Biochemical Characters in Downy Mildew Resistant and Susceptible Hybrids of Pearl Millet.Int.J.Curr.Microbiol.App.Sci. 7(5): 2590-2598. doi: https://doi.org/10.20546/ijcmas.2018.705.299