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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 558-563
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.061


Spectrum of Bacterial Isolates and their Antibiogram Isolated from Pus Sample
Deepali Shivajirao Kamble*, Dnyaneshwari P. Ghadage and Arvind V. Bhore
Department of Microbiology, Smt. Kashibai Navale, Medical College and General Hospital, Narhe, Pune-41, India
*Corresponding author
Abstract:

This study was conducted to know the spectrum of bacterial isolates from the pus  samples and their sensitivity pattern so that it will help the clinicians to treat patients and help the hospital to have its antibiotic policy. A total 221 pus samples were collected and were processed in the laboratory using standard microbiological procedures. The antibiotic sensitivity testing of all isolates was performed by Kirby Bauer’s disc diffusion method and results were  interpreted. Out of 221 pus samples 200 (90.49 %) samples yielded a positive culture whereas 21(9.50%) samples yielded no growth. Among 200 samples, 112 (56%) were male patients and 88 (44%) were female patients. The most predominant Gram positive cocci isolated was Staphylococcus aureus 52(27.65%) and predominant Gram negative bacteria was Pseudomonas spp 40 (21.27%) apart from other isolates such as Citrobacter spp12 (6.38%), Escherichia coli 24(12.76%), Klebsiella spp 28(14.89%), Proteus spp 12(6.38%), Methicillin resistant coagulase negative staphylococcus16 (8.51%) and Acinetobacter spp 16 (8.51%) were also isolated. ESBL was found in 28 (21.21%) and MRSA 15(28.84%). Gram positive cocci  shows 100% sensitivity to Vancomycin followed by Doxycycline. Whereas Gram negative bacilli were most susceptible to Imipenem followed by Amikacin and Gentamicin.


Keywords: Pus culture, Sensitivity, Resistance. Antibiogram, Disc diffusion method.
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How to cite this article:

Deepali Shivajirao Kamble, Dnyaneshwari P. Ghadage and Arvind V. Bhore. 2016. Spectrum of Bacterial Isolates and their Antibiogram Isolated from Pus Sample.Int.J.Curr.Microbiol.App.Sci. 5(8): 558-563. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.061