International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 538-544

Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains
Susmita Saha1, Jhinuk Basu Mullick1, Parichita Ray Choudhury1, Partha Saha1, Debaprasad Chakraborty2 and Samir Kumar Sil1*
1Molecular Genetics and Cell Physiology Lab., Department of Human Physiology, Tripura University, Suryamaninagar, West Tripura-799022, India
2Department of Pathology, Tripura Medical College, Hapania, West Tripura-799014, India
*Corresponding author

Parkia javanica, a medicinal plant, of North-East India, was screened for anti cholera activity against 4 Vibrio cholarae strains. Fresh stem barks of Parkia javanica was collected and crude methanol extract of Parkia javanica (MEPJ) was prepared by Hahnshin rotary evaporator. The minimum inhibitory concentration of MEPJ was evaluated on Vibrio cholarae strains by serial dilution technique. 10mg/ml of MEPJ is the IC100 dose for Vibrio cholarae strains. MEPJ also showed bactericidal activity against all four Vibrio cholarae at the same concentration. The lag phase become extended after treatment with IC50 dose of MEPJ. Intracellular reactive oxygen species (ROS) was increased (40% - 60%) in presence of MEPJ indicating ROS mediated mechanism of growth inhibition.

Keywords: Parkia javanica, Vibrio cholerae, growth curve, ROS.

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How to cite this article:

Susmita Saha, Jhinuk Basu Mullick, Parichita Ray Choudhury, Partha Saha, Debaprasad Chakraborty and Samir Kumar Sil. 2016. Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains.Int.J.Curr.Microbiol.App.Sci. 5(8): 538-544. doi:
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