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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 2036-2044
DOI: https://doi.org/10.20546/ijcmas.2018.705.239


Rapid Identification of M. tuberculosis Complex and Non Tuberculous Mycobacterium in Extra Pulmonary Tuberculosis using MGIT 320 Liquid Culture System and MPT64 Antigen Test
Mahwish Jawaid, Qursheed Sultana*, Ajaz Hussain, Md Khaleel and Maimoona Mustafa
Department of Microbiology, Deccan College of Medical Sciences, Hyderabad, India
*Corresponding author
Abstract:

Tuberculosis is a major cause of morbidity and mortality worldwide. Mycobacterium tuberculosis has infected one third of world's population and causes 8.8 million new cases with approximately 1.1 million deaths each year. A definitive diagnosis of TB can only be made by culturing Mycobacterium tuberculosis organisms from a specimen obtained from the patient. However diagnosing EPTB remains challenging because clinical samples obtained from relatively inaccessible sites may be paucibacillary, decreasing the sensitivity of diagnostic tests. The present study was initiated to determine the prevalence of Mycobacterium tuberculosis, Non Tuberculous Mycobacterium and its resistance to first line Anti-Tubercular drug from extra pulmonary samples. A total of 265 extra pulmonary samples were included in this study. All the samples screened by Zeihl-Neelsen AFB microscopy, were subjected to liquid culture using Mycobacterium Growth Indicator Tube (MGIT-320). Positive cultures were differentiated into Mycobacterium tuberculosis complex (MTBc) or Non-tubercular mycobacterium (NTM) by immunochromatography assay using MPT-64 antigen. Drug susceptibility testing of MTBc isolates done using MGIT-320. 32 MTBc and 7(NTM) grown in MGIT320 system were examined by the rapid MPT64 antigen detection. Among 32MTBc isolates 26 were sensitive to all the drugs and 6 were resistant to one or more drugs. Multiple drug resistant (MDR) isolates were 2. The susceptibility results were available within 11-13 days, thus within an average of 3 weeks of time complete results could be produced. We reported 6.6% prevalence of MDR-TB in EPTB at our tertiary care hospitals in southern India which is little lesser compared to other studies, reflecting better TB control programme. The prevalence of NTMs is also increasing, rising the importance of their recognition.


Keywords: Extrapulmonary tuberculosis, Mycobacterial growth indicator tube (MGIT), Mycobacterium tuberculosis complex (MTBc), Non-tubercular mycobacterium (NTM), MPT64 antigen test and multiple drug resistant (MDR)
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How to cite this article:

Mahwish Jawaid, Qursheed Sultana, Ajaz Hussain, Md Khaleel and Maimoona Mustafa. 2018. Rapid Identification of M. tuberculosis Complex and Non Tuberculous Mycobacterium in Extra Pulmonary Tuberculosis using MGIT 320 Liquid Culture System and MPT64 Antigen Test.Int.J.Curr.Microbiol.App.Sci. 7(5): 2036-2044. doi: https://doi.org/10.20546/ijcmas.2018.705.239