International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 529-537

Studies on Genetic Variability of Sucker Head, Garra gotyla (Fam: Cyprinidae) using Microsatellite Markers
Rakesh Matura1*, Rohit Kumar2, Veena Pande3 and Ashoktaru Barat2
1Wildlife Institute of India Post Box no 18 Chandrabani Dehradun Uttarakhand, India
2ICAR-Directorate of Coldwater Fisheries Research, Bhimtal-263 136, Nainital,Uttarakhand, India

3Department of Biotechnology, Kumaon University Nainital Uttarakhand India
*Corresponding author

Garra gotyla, commonly known as sucker head fish belongs to family Cyprinidae and distributed all along the mountain regions of India, Pakistan, Bangladesh and Myanmar. Different geographically isolated populations of the species were assessed using few microsatellite markers for future conservation and strategic breeding plan. Three wild populations of G. gotyla were analysed for genetic variability using 20 microsatellite markers. The mean observed heterozygosity ranged from 0.11 to 0.85. The mean no of alleles ranged from 3-11 with an average number of alleles per locus was 5.7 and were found to be in Hardy-Weinberg equilibrium (HWE) (p > 0.05). Polymorphism Information Content (PIC) ranged from 0.42 to 0.87. Overall Fst value of all the samples combined over all the microsatellite loci was found to be 0.173. The present genetic status of these three populations was also discussed for future application in genetic improvement of the species.

Keywords: Garra gotyla, Genetic variation, Null alleles, microsatellite, Polymorphism Information Content.

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How to cite this article:

Rakesh Matura, Rohit Kumar, Veena Pande and Ashoktaru Barat. 2016. Studies on Genetic Variability of Sucker Head, Garra gotyla (Fam: Cyprinidae) using Microsatellite MarkersInt.J.Curr.Microbiol.App.Sci. 5(8): 529-537. doi:
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