International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 525-528

Isolation and Sensitivity Pattern of Genital Candida Species in STD OP Female Attendees of Tertiary Care Hospital, Chennai, India
R. Vanaja1*, V. Sudha2 and Mangala Adisesh3
1Department of Microbiology, Madras Medical College, Chennai, India
2Department of Dermatology, ACS medical college, Chennai, India
 3Department of Microbiology, MMC & RGGGH, Chennai, India
*Corresponding author

The study was aimed to isolate Candida species from vaginal discharge of female patients with complaints of itching and to determine susceptibility pattern of vaginal isolates of Candida to antifungal drugs. A total of 400 vaginal discharge samples from all the female patients attending STD OP, Institute of Venereology, RGGGH / Madras Medical College, Chennai were included in this study. All the samples were subjected to KOH mount, cultured on Sabouraud’s Dextrose Agar, incubated overnight at room temperature, further gram stain and germ tube test were performed and subcultured on Hichrome Candida differentiation agar (M1297A). The species were differentiated by the morphology and colour of colonies. Antifungal susceptibility testing was done for all the species. Out of 400 samples, 83 patients had Candida isolations: C.glabrata – cream coloured colonies 37(45%), C.albicans – green coloured colonies 25(30%), C.tropicalis – blue coloured colonies 14(17%), C.krusei – pink coloured colonies 7(8%). All 83 isolates showed sensitivity to miconazole and nystatin, 39 to itraconazole, 6 to clotrimazole, 1 to fluconazole and nil with ketoconazole.

Keywords: Vaginal discharge, Candida,Hichrome agar, Nystatin and Micanazole.

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How to cite this article:

Vanaja, R., V. Sudha and Mangala Adisesh. 2016. Isolation and Sensitivity Pattern of Genital Candida Species in STD OP Female Attendees of Tertiary Care Hospital, Chennai, IndiaInt.J.Curr.Microbiol.App.Sci. 5(8): 525-528. doi:
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