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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 1898-1913
DOI: https://doi.org/10.20546/ijcmas.2018.705.223


Influence of Calpain Mediated Post-Mortem Ageing on Quality of Broiler Breeder Breast Fillets during Refrigerated Holding at (4 ± 1) °C
Anil Kumar Giri1, A.K. Biswas2, O.P. Dinani2*, K. Shrman3 and Nasir A. Mir2
1College of Agriculture, Waraseoni, Distt- Balaghat (M.P.), Pin- 481331, India 2ICAR Central Avian Research Institute, ICAR-IVRI, Izatnagar, Bareilly, UP-243122, India 3N.D.V.S.U.Jabalpur (M.P.), India
*Corresponding author
Abstract:

The aim of this study was to determine the activity of different calpains (µ and m) and calpastatin in broiler breeder breast fillets to understand their influence on ageing during holding at (4±1) °C. Both of the enzymes were extracted and they were subjected to casein zymography analysis. The purified enzymes were separated using anion exchange column chromatography, and their presence was confirmed by SDS-PAGE analysis. Casein Zymography results revealed the presence of µ-and m-calpains activity in breast fillets sample extracts. Though it has been observed that the band intensity keep on decreasing with the increasing ageing time which indicate the decrease in activity of these enzymes. The pH and Warner-Brazler Shear Force (WBSF) values were also decreased with the increase in ageing time while Lovibond tintometer colour, TBARS values, FFA contents and peroxide values were not much affected. Thus, it was concluded that both these enzymes are present in the muscle sample but that were autolyzed with the increase of aging. On the basis of results obtained it might be concluded that the optimum ageing time for broiler breeder breast fillets was optimized 24h at refrigerated (±1) °C holding.


Keywords: Calpain, Post-Mortem, Broiler Breeder Breast Fillets
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How to cite this article:

Anil Kumar Giri, A.K. Biswas, O.P. Dinani, K. Shrman and Nasir A. Mir. 2018. Influence of Calpain Mediated Post-Mortem Ageing on Quality of Broiler Breeder Breast Fillets during Refrigerated Holding at (4 ± 1) °C.Int.J.Curr.Microbiol.App.Sci. 7(5): 1898-1913. doi: https://doi.org/10.20546/ijcmas.2018.705.223