National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was to determine the activity of different calpains (µ and m) and calpastatin in broiler breeder breast fillets to understand their influence on ageing during holding at (4±1) °C. Both of the enzymes were extracted and they were subjected to casein zymography analysis. The purified enzymes were separated using anion exchange column chromatography, and their presence was confirmed by SDS-PAGE analysis. Casein Zymography results revealed the presence of µ-and m-calpains activity in breast fillets sample extracts. Though it has been observed that the band intensity keep on decreasing with the increasing ageing time which indicate the decrease in activity of these enzymes. The pH and Warner-Brazler Shear Force (WBSF) values were also decreased with the increase in ageing time while Lovibond tintometer colour, TBARS values, FFA contents and peroxide values were not much affected. Thus, it was concluded that both these enzymes are present in the muscle sample but that were autolyzed with the increase of aging. On the basis of results obtained it might be concluded that the optimum ageing time for broiler breeder breast fillets was optimized 24h at refrigerated (±1) °C holding.
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