International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 479-485

Study the Optimum Parameters for Production of Cloned L-Asparaginase Type I by Escherichia coli
Maha Hameed A. Al.Bahrani*
Molecular and Medical Biotechnology Department, College of Applied Biotechnology,
Al-Nahrin University, Baghdad, Iraq
*Corresponding author

In the present study, medium components and production conditions were different for carbon and nitrogen sources. The results have shown that the best carbon and nitrogen sources were lactose and yeast extract at concentration 1%. The different physiological factors include Temperature, pH, aeration and agitation. Besides, the results showed that the maximum production of L-Asparaginase was achieved after the incubation of the growth culture at 30°C and pH 7.Under these conditions, the activity of L-Asparaginase produced in culture medium was (10.3 IU/ml) and (10.5 IU/ml) respectively. The production of L-Asparaginase was modified during E.coli grown aerobically and when the shaker incubated at 220 rpm.

Keywords: L-Asparaginase,Escherichia coli,carbon and nitrogen sources

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How to cite this article:

Maha Hameed A. Al.Bahrani. 2016. Study the Optimum Parameters for Production of Cloned L-Asparaginase Type I by Escherichia coli.Int.J.Curr.Microbiol.App.Sci. 5(8): 479-485. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.