International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 436-445

Isolation and Characterization of Plant Growth Promoting Bacteria containing ACC Deaminase from Soil Collected from Central Himalayan Region of Uttarakhand, India
Dinesh Chandra* and A.K. Sharma
Department of Biological Sciences, CBS&H, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, U.S. Nagar, Uttarakhand, India
*Corresponding author

Bacteria possessing aminocyclopropane-1-carboxylate (ACC) deaminase activity reduce the level of stress induced ethylene production in plants conferring resistance and stimulating plants growth under various stressful conditions. A limited number of plant growth promoting bacteria (PGPB) possess the enzyme ACC deaminase (EC, a pyridoxal 5′-phosphate (PLP) dependent enzyme that catalyzes the cyclopropane ring fragmentation and deamination of ACC, the immediate precursor of ethylene in all higher plants into α-ketobutyrate and ammonia thereby lowering the level of plant ethylene. In this study, a total 297 bacteria were isolated from the 32 soil samples collected from the rainfed areas of Central Himalayan region of Kumaun, Uttarakhand, India. These soil bacteria were further screened for their ability to use ACC as a sole source of nitrogen in the DF salts minimal medium. In qualitative assay, 72 bacteria were found to be positive for ACC deaminase activity. Twenty eight bacterial isolates possessing the activity to cleave ACC were selected among the 72 isolates grown on the medium containing ACC as a sole nitrogen source were quantified and further characterized for different plant growth promoting traits Finally, 8 efficient bacterial isolates were selected on the basis of quantification of ACC deaminase activity and screened for drought tolerance using polyethylene glycol (PEG 8000). These selected bacteria withstand a substrate metric potential of -1.0 MPa (30 % PEG 8000) and therefore are considered to be drought-tolerant and could be helpful in eliminating the inhibitory effects caused by drought stress on the growth of plants.

Keywords: ACC deaminase, PGPB,stress,PEG 8000.Cyclopropane

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Dinesh Chandra and A.K. Sharma. 2016. Isolation and Characterization of Plant Growth Promoting Bacteria containing ACC Deaminase from Soil Collected from Central Himalayan Region of Uttarakhand, India.Int.J.Curr.Microbiol.App.Sci. 5(8): 436-445. doi:
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