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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 436-445
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.047


Isolation and Characterization of Plant Growth Promoting Bacteria containing ACC Deaminase from Soil Collected from Central Himalayan Region of Uttarakhand, India
Dinesh Chandra* and A.K. Sharma
Department of Biological Sciences, CBS&H, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145, U.S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

Bacteria possessing aminocyclopropane-1-carboxylate (ACC) deaminase activity reduce the level of stress induced ethylene production in plants conferring resistance and stimulating plants growth under various stressful conditions. A limited number of plant growth promoting bacteria (PGPB) possess the enzyme ACC deaminase (EC 4.1.99.4), a pyridoxal 5′-phosphate (PLP) dependent enzyme that catalyzes the cyclopropane ring fragmentation and deamination of ACC, the immediate precursor of ethylene in all higher plants into α-ketobutyrate and ammonia thereby lowering the level of plant ethylene. In this study, a total 297 bacteria were isolated from the 32 soil samples collected from the rainfed areas of Central Himalayan region of Kumaun, Uttarakhand, India. These soil bacteria were further screened for their ability to use ACC as a sole source of nitrogen in the DF salts minimal medium. In qualitative assay, 72 bacteria were found to be positive for ACC deaminase activity. Twenty eight bacterial isolates possessing the activity to cleave ACC were selected among the 72 isolates grown on the medium containing ACC as a sole nitrogen source were quantified and further characterized for different plant growth promoting traits Finally, 8 efficient bacterial isolates were selected on the basis of quantification of ACC deaminase activity and screened for drought tolerance using polyethylene glycol (PEG 8000). These selected bacteria withstand a substrate metric potential of -1.0 MPa (30 % PEG 8000) and therefore are considered to be drought-tolerant and could be helpful in eliminating the inhibitory effects caused by drought stress on the growth of plants.


Keywords: ACC deaminase, PGPB,stress,PEG 8000.Cyclopropane
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How to cite this article:

Dinesh Chandra and A.K. Sharma. 2016. Isolation and Characterization of Plant Growth Promoting Bacteria containing ACC Deaminase from Soil Collected from Central Himalayan Region of Uttarakhand, India.Int.J.Curr.Microbiol.App.Sci. 5(8): 436-445. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.047