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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(5): 539-550
DOI: https://doi.org/10.20546/ijcmas.2018.705.068


Effect of Chemical Treatment on Protein Profiling against Pre-Harvest Sprouting in Mung Bean
Priyanka Singh1, V.K. Chourasiya2 and R.D.S. Yadav1
1Department of Seed Science and Technology, N.D.U.A.T Kumarganj, Faizabad (U.P) 224229, India
2G.B.P.U.A.T Pantnagar (U.K) 263145, India
*Corresponding author
Abstract:

In the present study, SDS-Polyacrylamide gel electrophoresis was used to separate protein sub units of albumin and globulin under reducing conditions to study the induction or disappearance during inhibition of germination. The molecular masses of the albumin and globulin protein subunits were estimated in relation to the motilities of the standard molecular mass proteins (Bio-Rad). However the SDS PAGE resolution of seed albumin in Pusa vishal and K851 showed 9-13 band corresponding to 110-5.39kDa induced by coumarin followed by mallic hydrazide where as a low intensive band of molecular weight 42kDa was observed in control. All treatment exhibited more no of high molecular weight of protein band ranging 9.14-83.30kDa. The susceptibility was characterized by the presence of high water soluble albumin fraction and relatively low globulin content in samrat.


Keywords: Chemical, Protein, Pre-harvest sprouting
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How to cite this article:

Priyanka Singh, V.K. Chourasiya and Yadav, R.D.S. 2018. Effect of Chemical Treatment on Protein Profiling Against Pre-Harvest Sprouting in Mung Bean.Int.J.Curr.Microbiol.App.Sci. 7(5): 539-550. doi: https://doi.org/10.20546/ijcmas.2018.705.068