National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aims to identify strains of E.granulosus which infect human and intermediate hosts (sheep, cattle, buffaloes and camels) in Thi-Qar province by using PCR – RFLP technique. The molecular study is carried out on 75 hydatid cyst: include 25 human cyst were collected from Al-Hussein Imam Teaching Hospital. The samples of intermediate hosts hydatid cyst were collected from the abattoir of Nassiriyah municipality which included 25 cyst of sheep, 20 cyst of cattle, 3 cyst of buffaloes and 2 cyst of camels. The DNA is extracted from protoscolices of hydatid cysts isolated from infected organs. The PCR technique was used for amplification of rDNA – ITS1 gene. The PCR products isolated from different organs of human and others intermediate hosts were 1000 bp which is similar for sheep strain genotype 1. The results of PCR – RFLP analysis using Alu1 restriction enzyme with PCR product of ITS1 gene show two bands 800 bp and 200 bp while the size of bands by using Rsa1 restriction enzyme was 655 bp and 345 bp. Depending on PCR – RFLP patterns in all studied samples, it can be concluded that sheep strain G1 of E.granulosus is predominant in Thi-Qar province. Sequence analysis for ITS1 gene demonstrate the presence of sheep strain genotype 1 depending on sequences data for ITS1 gene in all samples which were similar 100 % for sheep strain in gene bank except one sample isolated from liver of an infected sheep which differs in position of nucleotide sequence and similar 99 % for sequence of sheep strain. The data base of this new strain is recorded in gene bank for DNA sequence in NCBI and ENA for the first time under the name Echinococcus granulosus strain WH1AW2 (GenBank: LT547814.1).
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