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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 309-315
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.033


Optimization of Fermentation Processes for Higher Poly Galacturonase Production from Various Soil Bacteria
Sanjay Patel1*, Dipika Pandya2 and S.A. Bhatt2
1Department of plant pathology, College of Agriculture, Navsari Agricultural University,
Bharuch Campus, Bharuch, India
2Department of Life sciences, Hemchandracharya North Gujarat University, Patan, India
*Corresponding author
Abstract:

Pectinases are the various hydrolytic and depolymerizing enzymes that breakdown pectin fibre. They have diverse application in field of food industry, pharmaceutical industry and waste management.  Pectinases are produced from almost all organisms including microbes, plant and animals. Pectinases from the fungal and bacterial origin is considerably more important for commercial production. Isolation of polygalacturonase producing bacterial species is carried out from the soil which is dumped with fruits and vegetables. The production of polygalacturonase by different soil isolates are optimized under submerged fermentation conditions. Nutritional and physical factors that influence polygalacturonase production are optimized by one factor at a time (OFAT) method in order to achieve high yield of polygalacturonase. In present study, production of polygalacturonase is enhanced by modifying various physic-chemical parameters. Different nitrogen sources and their varied concentration affect enzyme activities. Pectin concentration, pH of the medium, vitamins and amino acid concentration is also affect polygalacturonase production. For the assay of polygalacturonase enzyme polgalacturonic acid are used and the liberated galacturonic acid is measured by Di-Nitro Salicylic acid method. Among various nitrogen sources ammonium sulphate enhance polygalacturonase production and at slightly acidic pH enzyme activities is also observed higher.


Keywords: Pectinase, galacturonic acid, polygalacturonase (PGase), ammonium sulphate, urea, Submerged fermentation (SmF), solid-state fermentation (SSF).
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How to cite this article:

Sanjay Patel, Dipika Pandya and S.A. Bhatt. 2016. Optimization of Fermentation Processes for Higher Poly Galacturonase Production from Various Soil Bacteria.Int.J.Curr.Microbiol.App.Sci. 5(8): 309-315. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.033