National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pectinases are the various hydrolytic and depolymerizing enzymes that breakdown pectin fibre. They have diverse application in field of food industry, pharmaceutical industry and waste management. Pectinases are produced from almost all organisms including microbes, plant and animals. Pectinases from the fungal and bacterial origin is considerably more important for commercial production. Isolation of polygalacturonase producing bacterial species is carried out from the soil which is dumped with fruits and vegetables. The production of polygalacturonase by different soil isolates are optimized under submerged fermentation conditions. Nutritional and physical factors that influence polygalacturonase production are optimized by one factor at a time (OFAT) method in order to achieve high yield of polygalacturonase. In present study, production of polygalacturonase is enhanced by modifying various physic-chemical parameters. Different nitrogen sources and their varied concentration affect enzyme activities. Pectin concentration, pH of the medium, vitamins and amino acid concentration is also affect polygalacturonase production. For the assay of polygalacturonase enzyme polgalacturonic acid are used and the liberated galacturonic acid is measured by Di-Nitro Salicylic acid method. Among various nitrogen sources ammonium sulphate enhance polygalacturonase production and at slightly acidic pH enzyme activities is also observed higher.
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