International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 305-308

A Comparative Evaluation of Microbiological versus Histopathological Diagnostic Methods for Helicobacter pylori Infection
R.Deepa1*, Sujatha Varadarajan2, T.S. Vijayalakshmi3 and B. Natesan1
1Department of Microbiology, Institute of Microbiology, Madras Medical College and
RGGGH, Chennai-3, India
2King Institute of Preventive Medicine, Chennai, Tamilnadu 600032, India
3Department of Microbiology, Karpagavinayaga Institute of Medical Sciences, Kanchipuram, Tamilnadu 603308, India
*Corresponding author

Helicobacter pylori infection is a factor in the pathogenesis of gastroduodenal mucosal inflammation and gastric carcinoma. Though Histopathology plays a pivotal role in diagnosis of H.pylori infection, it has several limitations. This study was aimed at evaluating an in-house urease test and crush cytology for diagnosis of H. pylori infection. This cross sectional study was done for a period of three months on outpatients undergoing upper gastrointestinal endoscopy for various clinical indications. Antral biopsy specimens were obtained from 120 patients with findings of gastroduodenitis with or without gastric and duodenal ulcer. The samples were subjected to the following tests namely an in-house rapid urease test (RUT), Giemsa staining of crush cytology and histopathology. Kappa statistics was used to evaluate the various tests. RUT was positive in 45% of patients, Giemsa staining of crush cytology in 44.2% and histopathology in 45.8%. Considering histopathology report as the gold standard. Kappa statistics showed almost perfect degree  agreement of HPE with Giemsa staining of crush cytology (Kappa-0.96 and 98% agreement) and  RUT (Kappa 0.92 and 96% agreement). The sensitivity and specificity of Giemsa stain was 96.4% and 100% respectively and that of RUT was 94.5% and 96.9% respectively. Giemsa stain of crush cytology is a rapid and specific diagnostic test and is comparable to histopathology for the diagnosis of H. pylori infection.

Keywords: Helicobacter pylori, crush cytology,inhouse rapid urease test

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How to cite this article:

Deepa, R., Sujatha Varadarajan, T.S. Vijayalakshmi and Natesan, B. 2016. A Comparative Evaluation of Microbiological versus Histopathological Diagnostic Methods for Helicobacter pylori InfectionInt.J.Curr.Microbiol.App.Sci. 5(8): 305-308. doi:
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