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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 3499-3517
DOI: https://doi.org/10.20546/ijcmas.2018.704.396


Antibiotic Susceptibility of Lactobacillus sp. Isolated from Commercial Probiotic Products by E-Test Strip Method
Poonam Sharma1*, Santosh Anand1, Sudhir Kumar Tomar1* and Pawas Goswami2
1Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India
2Department of Microbiology, Bhaskaracharya College of Applied Sciences, Delhi University, New Delhi, India
*Corresponding author
Abstract:

Minimum Inhibitory Concentration (MIC) of 29 Lactobacillus isolates was determined by E-test strip method according to CLSI and EFSA, 2012 standards. All isolates displayed resistance towards aminoglycosides while 63% of isolates were found resistant to amikacin (MIC 16-256 µg/ml). Strains S8a and S8b (L. rhamnosus and L. acidophilus) were resistant to tetracycline (MIC 256 µg/ml). Isolates displayed resistance to quinolones [nalidixic acid with MIC 256 µg/ml; norfloxacin, ofloxacin, ciprofloxacin and levofloxacin with MIC 6-256 µg/ml; and sparofloxacin (MIC 0.25-4 µg/ml)], azolidiones [nitrofurantoin (MIC 3-256 µg/ml)] and cephalosporins (MIC 256µg/ml). Seventy-three percent of isolates displayed resistance against vancomycin while 66% to teicoplanin. Isolates, S1b (L. reutri), S4 (L. reuteri); S5 (L. plantarum); S8a (L. rhamnosus) and S8b (L. acidophilus) exhibited resistance towards beta-lactams. Very few strains exhibited susceptibility to class macrolides [roxithromycin (S10b- L. plantarum; MIC 0.5 µg/ml); clarithromycin (S6a- L. plantarum MIC 0.125 µg/ml); (S12- L. acidophilus MIC 0.25 µg/ml) and azithromycin (S12- L. acidophilus MIC 0.75 µg/ml)]. The present study found the prevalence of antibiotic resistance among commercial available probiotics, which may pose a safety risk among humans. Hence, antibiotic sensitivity should be considered as essential for the evaluation of safety assessment of probiotics.


Keywords: Antibiotic, Lactobacillus sp., E-test
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How to cite this article:

Poonam Sharma, Santosh Anand, Sudhir Kumar Tomar and Pawas Goswami. 2018. Antibiotic Susceptibility of Lactobacillus sp. Isolated from Commercial Probiotic Products by E-Test Strip Method.Int.J.Curr.Microbiol.App.Sci. 7(4): 3499-3517. doi: https://doi.org/10.20546/ijcmas.2018.704.396