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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 3167-3172
DOI: https://doi.org/10.20546/ijcmas.2018.704.359


Improvement of Colloidal and Temperature Stability of hsCRP Latex Reagents with Stabilizers
Praveena1*, C.V. Raghuveer2 and D.M. Vasudevan3
1Yenepoya university, Deralakatte, Mangalore, Karnataka - 575018, India
2Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka-563101, India
3Technical Director, Agappe, Cochin, Kerala - 683562, India
*Corresponding author
Abstract:

The role of high sensitivity C-reactive protein (hsCRP) level elevation in low-grade systemic inflammation was proved with the confirmation of cardiovascular risk assessment. Hence the measurement of hsCRP has got importance to predict the cardiovascular risk among the suspected population. The current market is still looking for a latex based reagent assay which requires minimum instrument setting and technical expertise to perform the test. Hence we have tried to develop a latex based test for hsCRP. The present study was conducted to test the colloidal and temperature stability of prepared hs-CRP latex reagents with different stabilizers. Trial reagent was tested for its colloidal stability using different stabilizing agents at a different temperature. A trial to improve colloidal stability of Bovine serum albumin (BSA) at higher temperature was studied using higher percentage w/v of BSA in the final reaction buffer. Latex reagent was tested for real-time temperature stability studies at three different temperatures and tested for colloidal stability and agglutination reaction with the positive control, with calibration concentration 1mg/L and 10 mg/L. The colloidal stabilising agent BSA showed higher colloidal stability, which has higher availability and cheaper in cost compared to other agents tested. Addition of higher concentration BSA causes inhibition of agglutination reaction with the CRP calibrator. The agglutination reaction with calibrator is showing a reduced reaction on reagent bottles kept at higher temperature. The colloidal stability is also seen reduced at a higher temperature. The concentration of BSA that can be used for further trials is estimated as 1.25% w/v, which gives better colloidal stability and visual agglutination reaction with the Hs CRP calibrators.


Keywords: Hs CRP, Stabilising agent, Colloidal stability, BSA, Temperature stability
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How to cite this article:

Praveena, C.V. Raghuveer and Vasudevan, D.M. 2018. Improvement of Colloidal and Temperature Stability of hsCRP Latex Reagents with Stabilizers.Int.J.Curr.Microbiol.App.Sci. 7(4): 3167-3172. doi: https://doi.org/10.20546/ijcmas.2018.704.359