Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2872-2880
DOI: https://doi.org/10.20546/ijcmas.2018.704.327


Screening of Bambara Groundnut (Vigna subterrenea Verdc. L.) Mutant Lines for Yellow Mosaic Virus Disease Resistance Using SSR Markers
Pranesh1, R. Nandini1, Kailash Chandra3 and N. Nagaraju2
1Department of Genetics and Plant Breeding, College of Agriculture, UAS, GKVK, Bengaluru-560065, Karnataka, India
2Department of Plant Pathology, UAS, Bengaluru, Karnataka, India
3Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India
*Corresponding author
Abstract:

Bambara groundnut (Vigna subterrenea Verdc. L) is an African pulse crop, cultivated mainly as protein source. The crop is most suitable for arid and semi-arid tropics and tolerant to saline soils. Low Productivity of pulses in the country is attributed to many biotic stresses which are ranging from plant ideotype to biotic and abiotic stresses. Among the various diseases, yellow mosaic virus (YMV) disease in pulses and also causes yield losses in Bambara groundnut. Hence the present study was conducted to screen 55 stabilized mutant lines of M6 generation irradiated with gamma irradiation. Five SSR primers were designed using the pick primer tool from the NCBI website for the RGA primer RGA 1F-CG/RGA 1R sequence reported in Vigna mungo due to its sequence similarity found in many of the Vigna species. Hence the same sequence was used to design primers. These heterologous primers were used to screen the isolated and stabilized mutants of Bambara groundnut. However all the designed primers were unable to amplify the mutant lines which infers that either all the lines lack resistance or these markers are not sufficient to identify the linked marker DNA to yellow mosaic virus disease resistance gene in Bambara groundnut. Hence an array of heterologus probes with large number of mutants needs to be screen.


Keywords: YMV, Mutation, Vigna subterrenea Verdc. L, Heterologous probes
Download this article as Download

How to cite this article:

Pranesh, R. Nandini, Kailash Chandra and Nagaraju, N. 2018. Screening of Bambara Groundnut (Vigna subterrenea Verdc. L.) Mutant Lines for Yellow Mosaic Virus Disease Resistance Using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 7(4): 2872-2880. doi: https://doi.org/10.20546/ijcmas.2018.704.327