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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2747-2755
DOI: https://doi.org/10.20546/ijcmas.2018.704.313


Evaluation of Procalcitonin, CRP and Blood Culture in the Diagnosis of Neonatal Sepsis
Rashmi Padmanabha Mahale, Morubagal Raghavendra Rao* and Sowmya Govindanahalli Shivappa
Department of Microbiology, JSS Medical College, Jss academy of Higher Education and Research, Shivarathreswara Nagar Mysore-570015, Karnataka, India
*Corresponding author
Abstract:

Sepsis is a major cause of morbidity and mortality among neonates. Neonatal sepsis may be categorized as early-onset or late-onset. Clinical manifestations of neonatal sepsis are non-specific, therefore, clinical diagnosis of sepsis is difficult and laboratory help is required. Present study was done to evaluate three different methods in diagnosis of neonatal sepsis. 3 ml of venous blood was collected employing strict aseptic precautions from all babies admitted to the NICU in our hospital during the study period. 2 ml of blood was aseptically added to BacT/ ALERT PF culture bottle. Bacteria grown from these bottles were identified by using the standard protocol. The antibiotic susceptibility for the isolates was tested by Kirby Bauer method following CLSI guidelines. Remaining blood sample was used for PCT estimation by immunochromatography assay and CRP estimation by chemiluminescence method. 50 blood samples were collected from neonates admitted to the NICU. The most common risk factor of suspected septicaemia in our study was birth asphyxia (32%), followed by preterm birth (28%). Blood culture was positive in 12 (24%) cases. The most common etiological agent was Staphylococcus aureus. PCT was positive in all blood culture positive (proven sepsis) cases. Out of 50 samples tested for CRP, 33(66%) were positive. A wide range of CRP values were obtained, ranging from 1.83 mg/dL to 120 mg/dL. Out of the 34 PCT positive cases, 33 (97.05%) were also CRP positive. Blood culture even though considered as gold standard it is time consuming and at times gives false negative results due to administration of antibiotics. PCT and CRP are reliable markers which aid in diagnosis of neonatal sepsis, which have the same diagnostic accuracy. CRP, when compared to PCT is affordable and can be conveniently used as a marker for the diagnosis of neonatal sepsis, especially in developing countries with poor resources


Keywords: Blood culture, Neonatal sepsis, C-reactive protein, Procalcitonin
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How to cite this article:

Rashmi Padmanabha Mahale, Morubagal Raghavendra Rao and Sowmya Govindanahalli Shivappa. 2018. Evaluation of Procalcitonin, CRP and Blood Culture in the Diagnosis of Neonatal Sepsis.Int.J.Curr.Microbiol.App.Sci. 7(4): 2747-2755. doi: https://doi.org/10.20546/ijcmas.2018.704.313