National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aimed to use plant biotechnology techniques and biochemical analyses to achieve promising procedures for regenerated shootlets of Hypericum sinaicum L. via direct/ indirect regeneration. So and related to this concern, this investigation was carried to examine the effect of different explant types and culture conditions, with emphasis on the type and concentrations of plant growth regulators (PGRs) in culture media on shootlets regeneration and prolonged maintenance of in vitro material. The results showed that, direct/indirect shoot formation were induced by all levels of PGRs but the optimum medium for direct shootlets induction and multiplication was MS supplemented with 0.1 mg/l NAA combination with 1mg/l BA compared with other supplementations. The maximum number of direct shootlets regeneration (24 shootlets per explant) was recorded with nodal explant cultured on MS medium supplemented with 0.1 NAA mg/l plus 1mg/l BA under light condition. While the maximum number of indirect shootlets regeneration (14 shootlets per callus) was recorded with the same previous explant cultured on MS medium augmented with 0.5 mg/l TDZ and 0.05 mg/l NAA under light and darkness condition. In this regard, after regeneration was developed, the vegetative growth dynamics patterns were estimated through the following parameters; number of shoots/one piece of callus, length of shoot (cm), fresh weight (g/jar), dry weight (g/jar), dry matter content (%). In addition, determination of hypericin component in in vitro germinated plantlet(s) as control and regenerated shoot was estimated using high performance liquid chromatography (HPLC) techniques. The highest value of hypericin contents 84.70, 72.18 µg/g dry weights were estimated in direct shoots regeneration derived from nodal and root explants on MS supplemented with 0.1 mg/l NAA+ 1 mg/l BA at light condition.
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