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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 182-196
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.020


Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin Content
Heba D. Khlifa1, Ibrahim A. Ibrahim2, M. Bekhit2, M. Szkop3 and H.S. Taha1*
 1Plant Biotechnology Department, National Research Centre (NRC), Dokki, P.O.12622Cairo Egypt
2Plant Biotechnology Dept., Genetic Engineering Institute (GEBRI),University of Sadat City, Egypt

3Biochemistry Department, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Poland
*Corresponding author
Abstract:

The present study aimed to use plant biotechnology techniques and biochemical analyses to achieve promising procedures for regenerated shootlets of Hypericum sinaicum L. via direct/ indirect regeneration. So and related to this concern, this investigation was carried to examine the effect of different explant types and culture conditions, with emphasis on the type and concentrations of plant growth regulators (PGRs) in culture media on shootlets regeneration and prolonged maintenance of in vitro material. The results showed that, direct/indirect shoot formation were induced by all levels of PGRs but the optimum medium for direct shootlets induction and multiplication was MS supplemented with 0.1 mg/l NAA combination with 1mg/l BA compared with other supplementations. The maximum number of direct shootlets regeneration (24 shootlets per explant) was recorded with nodal explant cultured on MS medium supplemented with 0.1 NAA mg/l plus 1mg/l BA under light condition. While the maximum number of indirect shootlets regeneration (14 shootlets per callus) was recorded with the same previous explant cultured on MS medium augmented with 0.5 mg/l TDZ and 0.05 mg/l NAA under light and darkness condition. In this regard, after regeneration was developed, the vegetative growth dynamics patterns were estimated through the following parameters; number of shoots/one piece of callus, length of shoot (cm), fresh weight (g/jar), dry weight (g/jar), dry matter content (%). In addition, determination of hypericin component in in vitro germinated plantlet(s) as control and regenerated shoot was estimated using high performance liquid chromatography (HPLC) techniques. The highest value of hypericin contents 84.70, 72.18 µg/g dry weights were estimated in direct shoots regeneration derived from nodal and root explants on MS supplemented with 0.1 mg/l NAA+ 1 mg/l BA at light condition.


Keywords: Hypericum  sinaicum,in vitro propagation, regeneration, multiplication, hypericin.
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How to cite this article:

Heba D. Khlifa, Ibrahim A. Ibrahim, M. Bekhit, M. Szkop and H.S. Taha. 2016. Hypericum sinaicum L. in Vitro Regeneration and Analysis of Hypericin ContentInt.J.Curr.Microbiol.App.Sci. 5(8): 182-196. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.020