National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Patients colonized with cabapenem resistant Enterobacteriaece(CRE) especially gastrointestinal carriers are source of transmission in healthcare setting . This emphasizes the need for a sensitive screening procedure to identify these micro-organisms to the success of infection control measures. Our aim was to assess different agar-based screening methods for detection of colonization with Klebsiella Pneumoniae Carbapenemase (KPC) producing Carbapenem- Resistant Enterobacteriaece (CRE) in rectal swabs of geriatric intensive care unit patients. Rectal swabs from 100 patients were collected. All 100 swabs were subcultered on MacConkey agar supplemented with 1μg/ml imipenem (Mac IPM), Mac. agar supplemented with 1μg/ml ertapenem (Mac ETP), and MacConkey agar with 10μg discs of imipenem, meropenem and ertapenem (Mac disks) and detection of blaKPC gene by polymerase chain reaction (PCR). 74% of rectal swabs were positive by PCR. Mac ETP showed the highest sensitivity for detection of KPC producing CRE 71% followed by Mac. Disks 66%, and Mac.IPM 65%. In conclusion the Mac ETP is the most reliable, sensitive, rapid and cost-effective screening media for detection of KPC – producing CRE from rectal swabs for good implementation of infection control measures.
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