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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(8): 176-181
DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.019


Screening Agars for Detection of Klebsiella pneumoniae Carbapenemase -Carbapenem Resistant Enterobacteriaceae from Rectal Swabs of ICU Patients
Hala B. Othman1, Dalia H. Abd El Hamid1*, Hoda Ezz El –arab Abdul-wahab1, Enjy Alaa Eldin1 and Walaa W. Aly2
1Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Egypt
2Department of Geriatric and Gerontology, Faculty of Medicine, Ain Shams University, Egypt
*Corresponding author
Abstract:

Patients colonized with cabapenem resistant  Enterobacteriaece(CRE) especially gastrointestinal carriers   are source of transmission in  healthcare setting . This emphasizes the need for a sensitive screening procedure to identify these micro-organisms to the success of infection control measures. Our aim was to assess different agar-based screening methods for detection of colonization with Klebsiella Pneumoniae Carbapenemase (KPC) producing Carbapenem- Resistant Enterobacteriaece (CRE) in rectal swabs of geriatric intensive care unit patients. Rectal swabs from 100 patients were collected. All 100 swabs were subcultered on MacConkey agar supplemented with 1μg/ml imipenem (Mac IPM), Mac. agar supplemented with 1μg/ml ertapenem (Mac ETP), and MacConkey agar with 10μg discs of imipenem, meropenem and ertapenem (Mac disks) and detection of  blaKPC gene by polymerase chain reaction (PCR). 74% of rectal swabs were positive  by PCR. Mac ETP showed the highest sensitivity for detection of KPC producing CRE 71% followed by  Mac. Disks 66%, and Mac.IPM 65%. In conclusion the Mac ETP is the most reliable, sensitive, rapid and cost-effective  screening  media for detection of KPC – producing  CRE from rectal swabs  for good implementation of infection control measures.


Keywords: Carbapnem Resistant Enterobacteriaece (CRE), KPC, Carbapenemase, PCR, blaKPC gene, Carbapenem.
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How to cite this article:

Hala B. Othman, Dalia H. Abd El Hamid, Hoda Ezz El –arab Abdul-wahab, Enjy Alaa Eldin and Walaa W. Aly. 2016. Screening Agars for Detection of Klebsiella pneumoniae Carbapenemase -Carbapenem Resistant Enterobacteriaceae from Rectal Swabs of ICU Patients.Int.J.Curr.Microbiol.App.Sci. 5(8): 176-181. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.019